CD107 antigen like family member A; CD107 antigen-like family member A; CD107a; CD107a antigen; LAMP 1; LAMP-1; LAMP1_HUMAN; LAMPA; LGP120; lgpA; Lysosomal membrane glycoprotein 120KD; Lysosomal Associated Membrane Protein 1; Lysosome associated membrane
Cat:
SLM51301M
Species Reactivity：
Human,
Immunogen：
KLH conjugated synthetic peptide derived from human LAMP1
Format：
Liquid
Storage instructions：
Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Concentration：
1mg/ml
Clonality：
Monoclonal
Isotype：
IgG1,k
Applications：
WB=1:500-1000ELISA=1:5000-10000ICC=1:20-200IF=1:20-200not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.
Host：
Mouse
Product Overview：
Sample: Lane 1: Human HT1080 cell lysates Lane 2: Human Hela cell lysates Lane 3: Human U937 cell lysates Primary: Anti-LAMP1 (SLM51301M) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilutionPredicted band size: 42 kDaObserved band size: 90-120 kDa
Lysosome associated membrane protein (LAMP1), also known as lgp120 or lgpA, is a type 1 integral membrane protein that is transported from trans Golgi networks to endosomes and then lysosomes. Upon cell activation, LAMP1 transfer to the plasma membrane is dependent on a carboxyl terminal tyrosine based motif (YXXI). Perturbation in the spacing between the tyrosine based motif relative to the membrane abolishes lysosome localization of LAMP1. This mutant protein then cycles between the plasma membrane and the endosome. Cell surface LAMP1 and LAMP2 have been shown to promote adhesion of human peripheral blood mononuclear cells (PBMC) to vascular endothelium, therefore they are possibly involved in the adhesion of PBMCs to the site of inflammation.
Function: Presents carbohydrate ligands to selectins. Also mplicated in tumor cell metastasis.
Subcellular Location: Cell membrane; Single-pass type I membrane protein. Endosome membrane; Single-pass type I membrane protein. Lysosome membrane; Single-pass type I membrane protein. Note=This protein shuttles between lysosomes, endosomes, and the plasma membrane.
Post-translational modifications: O- and N-glycosylated; some of the 18 N-linked glycans are polylactosaminoglycans.
Sample:
Lane 1: Human HT1080 cell lysates
Lane 2: Human Hela cell lysates
Lane 3: Human U937 cell lysates
Primary: Anti-LAMP1 (SLM51301M) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 42 kDa
Observed band size: 90-120 kDa
