Mouse Anti-GFP antibody | Sunlong Medical

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Green fluorescence protein;

Cat:

SLM51005M

Species Reactivity：

Recombinant protein

Immunogen：

Recombinant Green Fluorescence Protein

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Monoclonal

Isotype：

IgG1

Applications：

WB=1:5000-20000ELISA=1:5000-20000IF=1:20-200not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Mouse

Product Overview：

Sample: GFP-Tagged Fusion Protein Overexpression E.coli Lysate (Cat#: SL33009P) at 2 ug Primary: Anti-GFP-Tag (SLM51005M) at 1/2000 ~ 1/10000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 28 kD Observed band size: 28 kD Sample: GFP-Tagged Fusion Protein Overexpression E.coli Lysate (Cat#: SL33009P) at 4 ug Primary: Anti-GFP-Tag (SLM51005M) at 1/2000 ~ 1/10000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 28 kD Observed band size: 28 kD Sample: Lane 1: GFP-Tagged fusion protein (full length) Overexpression E.coli Lysate (Cat#: SL33009P) at 4 ugPrimary: Anti-GFP-Tag (SLM51005M) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilutionPredicted band size: 28 kDObserved band size: 28 kD

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Unit:

Price: $316.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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Green fluorescence protein (GFP) is a 27 kDa protein derived from the jellyfish Aequorea victoria, which emits green light (emission peak at a wavelenth of 509 nm) when excited by blue light (excitation peak at a wavelenth of 395 nm). Green Fluorescent Protein (GFP) has become an invaluable tool in cell biology research, since its intrinsic fluorescence can be visualized in living cells. GFP fluorescence is stable under fixation conditions and suitable for a variety of applications. GFP has been widely used as a reporter for gene expression, enabling researchers to visualize and localize GFP-tagged proteins within living cells without the need for chemical staining. Other applications of GFP include assessment of protein protein interactions through the yeast two hybrid system and measurement of distance between proteins through fluorescence energy transfer (FRET) protocols. GFP technnology has considerably contributed to a greater understanding of cellular physiology.

Function:
Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca(2+)-activated photoprotein aequorin.

Subunit:
Monomer.

Tissue Specificity:
Photocytes.

Post-translational modifications:
Contains a chromophore consisting of modified amino acid residues. The chromophore is formed by autocatalytic backbone condensation between Ser-65 and Gly-67, and oxidation of Tyr-66 to didehydrotyrosine. Maturation of the chromophore requires nothing other than molecular oxygen.

Similarity:
Belongs to the GFP family.

SWISS:
P42212

Gene ID:
107331116

Picture

Sample:
GFP-Tagged Fusion Protein Overexpression E.coli Lysate (Cat#: SL33009P) at 4 ug
Primary: Anti-GFP-Tag (SLM51005M) at 1/2000 ~ 1/10000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 28 kD
Observed band size: 28 kD

Sample:
Lane 1: GFP-Tagged fusion protein (full length) Overexpression E.coli Lysate (Cat#: SL33009P) at 4 ug
Primary: Anti-GFP-Tag (SLM51005M) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 28 kD
Observed band size: 28 kD

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