Mouse Anti-PINK1 antibody | Sunlong Medical

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PINK1_HUMAN; PTEN Induced Putative Kinase 1; PTEN-Induced Putative Kinase Protein 1; BRPK; Serine/Threonine-Protein Kinase PINK1, Mitochondrial; Parkinson Disease (Autosomal Recessive) 6; Protein Kinase BRPK; EC 2.7.11.1; PARK6;

Cat:

SLM51265M

Species Reactivity：

(predicted: Human,Mouse,)

Immunogen：

Recombinant human PINK1 protein

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Monoclonal

Isotype：

IgG

Applications：

WB=1:200-1000IHC-P=1:50-100IHC-F=1:50-100ICC=1:25IF=1:25（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Mouse

Product Overview：

All lanes : Anti-Pink1(115-213) at 1:800 dilution Lane 1:A431 whole cell lysate Lane 2: Mouse brain lysateLysates/proteins at 20 μg per lane. Secondary GoatAnti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000dilution. Predicted band size : 63 kDa Blocking/Dilutionbuffer: 5% NFDM/TBST.

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Unit:

Price: $316.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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This gene encodes a serine/threonine protein kinase that localizes to mitochondria. It is thought to protect cells from stress-induced mitochondrial dysfunction. Mutations in this gene cause one form of autosomal recessive early-onset Parkinson disease. [provided by RefSeq, Jul 2008]

Function:
Protects against mitochondrial dysfunction during cellular stress by phosphorylating mitochondrial proteins. Involved in the clearance of damaged mitochondria via selective autophagy (mitophagy) by mediating activation and translocation of PRKN . Targets PRKN to dysfunctional depolarized mitochondria through the phosphorylation of MFN2 . Activates PRKN in 2 steps: (1) by mediating phosphorylation at 'Ser-65' of PRKN and (2) mediating phosphorylation of ubiquitin, converting PRKN to its fully-active form . Required for ubiquinone reduction by mitochondrial complex I by mediating phosphorylation of complex I subunit NDUFA10 (By similarity).

Subunit:
Interacts with PRKN. Interacts with FBXO7. Forms a complex with PRKN and PARK7.

Subcellular Location:
Localizes mostly in mitochondrion and the 2 proteolytic processed fragments of 55 kDa and 48 kDa localize mainly in cytosol.

Tissue Specificity:
Highly expressed in heart, skeletal muscle and testis, and at lower levels in brain, placenta, liver, kidney, pancreas, prostate, ovary and small intestine. Present in the embryonic testis from an early stage of development.

Post-translational modifications:
Autophosphorylation at Ser-228 and Ser-402 is essential for Parkin/PRKN recruitment to depolarized mitochondria.
Two shorter forms of 55 kDa and 48 kDa seem to be produced by proteolytic cleavage and localize mainly in cytosol.

Similarity:
Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.

SWISS:
Q9BXM7

Gene ID:
65018

Database links:

Entrez Gene: 65018 Human

Entrez Gene: 68943 Mouse

Entrez Gene: 298575 Rat

SwissProt: Q9BXM7 Human

SwissProt: Q99MQ3 Mouse

Picture

All lanes : Anti-Pink1(115-213) at 1:800 dilution Lane 1: A431 whole cell lysate Lane 2: Mouse brain lysate Lysates/proteins at 20 μg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 63 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

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