Rabbit Anti-CKAP1/FITC Conjugated antibody

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TBCB; CG22; CKAPI; Cytoskeleton associated protein 1; Cytoskeleton associated protein CKAPI; Cytoskeleton-associated protein 1; Cytoskeleton-associated protein CKAPI; TBCB_HUMAN; Tubulin folding cofactor B; Tubulin specific chaperone B; Tubulin-folding co

Cat:

SL11713R-FITC

Species Reactivity：

(predicted: Human,Mouse,Rat,Dog,Horse,Rabbit,)

Immunogen：

KLH conjugated synthetic peptide derived from human CKAP1 (1-100aa)

Format：

Lyophilized or Liquid

Storage instructions：

Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of ant

Buffer：

0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

ICC=1:50-200IF=1:50-200not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Calculated MW：

27kDa

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Unit:

Price: $596.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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background:
Microtubules, the primary component of the cytoskeletal network, are highly dynamic structures composed of Alpha/Beta Tubulin heterodimers. Biosynthesis of functional microtubules involve the participation of several chaperones, termed Tubulin folding cofactors A (TBCA), B (TBCB), D (TBCD), E (TBCE) and C (TBCC), that act on folding intermediates downstream of the cytosolic chaperon, alternatively named TCP. TBCB (tubulin folding cofactor B), also known as CG22, CKAP1 or CKAPI, is a 244 amino acid cytoplasmic protein containing one CAP-Gly domain and in widely expressed. TBCB is involved in the regulation of tubulin heterodimer dissociation and may function as a negative regulator of axonal growth.

Function:
Binds to alpha-tubulin folding intermediates after their interaction with cytosolic chaperonin in the pathway leading from newly synthesized tubulin to properly folded heterodimer. Involved in regulation of tubulin heterodimer dissociation. May function as a negative regulator of axonal growth.

Subunit:
Supercomplex made of cofactors A to E. Cofactors A and D function by capturing and stabilizing tubulin in a quasi-native conformation. Cofactor E binds to the cofactor D-tubulin complex; interaction with cofactor C then causes the release of tubulin polypeptides that are committed to the native state. Cofactors B and E can form a heterodimer which binds to alpha-tubulin and enhances their ability to dissociate tubulin heterodimers. Binds to GAN.

Subcellular Location:
Cytoplasm. Cytoplasm > cytoskeleton. Colocalizes with microtubules. In differentiated neurons, located in the cytoplasm. In differentiating neurons, accumulates at the growth cone.

Tissue Specificity:
Found in most tissues.

Post-translational modifications:
Phosphorylation by PAK1 is required for normal function. Phosphorylated upon DNA damage, probably by ATM or ATR.
Ubiquitinated in the presence of GAN which targets it for degradation by the proteasome.

Similarity:
Belongs to the TBCB family.
Contains 1 CAP-Gly domain.

Database links:

Entrez Gene: 1155 Human

Entrez Gene: 66411 Mouse

Entrez Gene: 292777 Rat

Omim: 601303 Human

SwissProt: Q99426 Human

SwissProt: Q9D1E6 Mouse

Unigene: 31053 Human

Unigene: 27947 Mouse

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

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