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Home > Product > Antibody > Rabbit Anti-GRC5/FITC Conjugated antibody
CENP-35; GRC5; PHF2; JHDM1E; Jumonji C domain containing histone demethylase 1E; jumonji containing protein; Lysine-specific demethylase PHF2; PHD finger protein 2; Phf2; PHF2_HUMAN.
Cat:
SL11659R-FITC
Species Reactivity：
Human,(predicted: Mouse,Rat,Chicken,Horse,Sheep,)
Immunogen：
KLH conjugated synthetic peptide derived from human GRC5/PHF2 (1001-1096aa)
Format：
Lyophilized or Liquid
Storage instructions：
Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of ant
Buffer：
0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Concentration：
1mg/ml
Clonality：
Polyclonal
Isotype：
IgG
Applications：
ICC=1:50-200IF=1:50-200not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.
Host：
Rabbit
Calculated MW：
121kDa
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Product PDFs
Datasheet:


background:
PHF2 is a 1,101 amino acid protein belonging to the PHD finger protein family. Members of the PHD finger protein family function as transcriptional regulators that affect gene expression by modulating chromatin structure. Expressed in a wide variety of tissues, PHF2 localizes to the nucleus and contains one PHD-type zinc finger and one JMJC domain, suggesting a possible role for PHF2 in transcription regulation and chromatin remodeling. The gene encoding PHF2 lies in the candidate region for hereditary sensory neuropathy type I (HSN1), a disorder characterized by sensory dysfunction. PHF2 exhists as two isoforms produced by alternative splicing, designated isoform 1 and 2 respectively.

Function:
Lysine demethylase that demethylates both histones and non-histone proteins. Enzymatically inactive by itself, and becomes active following phosphorylation by PKA: forms a complex with ARID5B and mediates demethylation of methylated ARID5B. Demethylation of ARID5B leads to target the PHF2-ARID5B complex to target promoters, where PHF2 mediates demethylation of dimethylated 'Lys-9' of histone H3 (H3K9me2), followed by transcription activation of target genes. The PHF2-ARID5B complex acts as a coactivator of HNF4A in liver. PHF2 is recruited to trimethylated 'Lys-4' of histone H3 (H3K4me3) at rDNA promoters and promotes expression of rDNA.

Subunit:
Component of the PHF2-ARID5B complex, at least composedof PHF2 and ARID5B. Interacts with HNF4A and NR1H4.

Subcellular Location:
Nucleus, nucleolus.

Tissue Specificity:
Widely expressed, including in liver (at protein level).

Post-translational modifications:
Phosphorylated by PKA on specific serine residues, leading to the formation of an active lysine demethylase complex.

Similarity:
Belongs to the JHDM1 histone demethylase family. JHDM1D subfamily.
Contains 1 JmjC domain.
Contains 1 PHD-type zinc finger.

Database links:

Entrez Gene: 5253 Human

Entrez Gene: 18676 Mouse

Entrez Gene: 306814 Rat

Omim: 604351 Human

SwissProt: O75151 Human

SwissProt: Q9WTU0 Mouse

  • Unigene: 212554 Mouse


    • Important Note:
    This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
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