background:
SRGAPs contain a highly conserved overall primary structure and play an important role in the cell facilitating Slit-robo signaling in cell migration and axon guidance. SRGAP1 (Slit-robo Rho GTPase activating protein 1), also known as ARHGAP13 (Rho GTPase activating protein 13), functions as a GTPase-activating protein for Cdc42 and Rho A. Expressed in kidney, testis, lung and brain, SRGAP1 contains an FCH (Fes/CIP4 homology) domain, a Rho-GAP domain and an SH3 domain. In the presence of Slit, SRGAP1 (via its SH3 domain) binds to the CC3 motif in robo (a protein responsible for mediating the repulsive effect of Slit) with higher affinity and inhibits Cdc42 activity in a robo/SRGAP-dependent manner. More specifically, SRGAP1 increases the intrinsic GTPase activity of Cdc42, thereby converting it to its inactive, GDP-bound form. Inactivation of Cdc42 ultimately leads to a decrease in actin polymerization.
Function:
GTPase-activating protein for RhoA and Cdc42 small GTPases. Together with CDC42 seems to be involved in the pathway mediating the repulsive signaling of Robo and Slit proteins in neuronal migration. SLIT2, probably through interaction with ROBO1, increases the interaction of SRGAP1 with ROBO1 and inactivates CDC42.
Subunit:
Homodimer (Probable). Forms heterooligomer with SRGAP2 and SRGAP3 through its F-BAR domain. Interacts with ROBO1, CDC42 and RHOA. Interacts with FASLG.
Tissue Specificity:
Expressed in brain, lung, kidney, and testis.
Similarity:
Contains 1 FCH domain.
Contains 1 Rho-GAP domain.
Contains 1 SH3 domain.
Database links:
Entrez Gene: 57522 Human
Entrez Gene: 117600 Mouse
Entrez Gene: 314903 Rat
Omim: 606523 Human
SwissProt: Q7Z6B7 Human
SwissProt: Q91Z69 Mouse
Unigene: 210751 Human
Unigene: 593803 Human
Unigene: 288698 Mouse
Unigene: 208903 Rat
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
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