Rabbit Anti-SIM1/FITC Conjugated antibody

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bHLHe14; Class E basic helix-loop-helix protein 14; SIM1; SIM1_HUMAN; Single minded homolog 1; Single-minded homolog 1.

Cat:

SL11585R-FITC

Species Reactivity：

(predicted: Human,Mouse,Rat,Chicken,Dog,Pig,Cow,Horse,Rabbit,Zebrafish,)

Immunogen：

KLH conjugated synthetic peptide derived from human SIM1 (2-80aa)

Format：

Lyophilized or Liquid

Storage instructions：

Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of ant

Buffer：

0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

ICC=1:50-200IF=1:50-200not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Calculated MW：

85kDa

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Unit:

Price: $596.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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background:
The Per-Arnt-Sim (PAS) domain was identified as a 270 amino acid motif that mediates associations between various PAS family transcription factors. Several PAS domain family members have been identified including AhR, Arnt 1, and single-minded proteins (SIM1 and SIM2). The aromatic (aryl) hydrocarbon receptor, AhR, is a ligand dependent transcription factor that interacts with specific DNA sequences termed xenobiotic responsive elements (XREs) to activate several genes including CYP1A1, glutathione S-transferase Ya subunit and DT-diaphorase. The Ah receptor nuclear translocator protein 1 (Arnt 1) is required for ligand-dependent nuclear translocation of the Ah receptor and is also necessary for Ah receptor binding to the XRE element. Both SIM1 and SIM2 inhibit AhR/Arnt dimerization, thus inhibiting transcriptional activation. The SIM genes are thought to be involved in the directing and regionalization of tissues during development and the SIM2 gene, which is located on chromosome 21, is a candidate for the gene responsible for Down syndrome.

Function:
Transcriptional factor that may have pleiotropic effects during embryogenesis and in the adult.

Subunit:
Efficient DNA binding requires dimerization with another bHLH protein. Heterodimer of SIM1 and ARNT.

Subcellular Location:
Nucleus.

Similarity:
Contains 1 basic helix-loop-helix (bHLH) domain.
Contains 1 PAC (PAS-associated SLCterminal) domain.
Contains 2 PAS (PER-ARNT-SIM) domains.
Contains 1 Single-minded SLCterminal domain.

Database links:

Entrez Gene: 6492 Human

Entrez Gene: 20464 Mouse

Entrez Gene: 309888 Rat

Omim: 603128 Human

SwissProt: P81133 Human

SwissProt: Q61045 Mouse

Unigene: 520293 Human

Unigene: 4774 Mouse

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

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