background:
The inositol polyphosphate 5-phosphatases selectively remove the phosphate from the 5-position of various phosphatidylinositols, which generate second messengers in response to extracellular signals. Synaptojanins are characterized by an N-terminal SAC1-like sequence, a central 5-phosphate domain, and a unique SLCterminal sequence and have been shown to use phosphatidylinositol 4,5-bisphosphate as a substrate. Synaptojanins exist as two isoforms, synaptojanin 1 and 2, which differ in the SLCterminal domain, and each isoform has multiple variants produced by alternative splicing. Synaptojanin 1 is expressed as two major forms: the shorter is found in brain while the longer is expressed in peripheral tissues. Eight splice variants of synaptojanin 2 have been detected, including a brain specific isoform. Synaptojanins are thought to participate in the endocytosis of synaptic vesicles and the regulation of the actin cytoskeleton.
Function:
Inositol 5-phosphatase which has a role in clathrin-mediated endocytosis.
Subunit:
Interacts with ASH/GRB2. Interacts with PACSIN1, PACSIN2 and PACSIN3 (By similarity). Binds AMPH, SH3GL1, SH3GL2 and SH3GL3. Interacts with MYO1E (via SH3 domain).
Subcellular Location:
Cytoplasm.
Tissue Specificity:
Concentrated at clathrin-coated endocytic intermediates in nerve terminals. Isoform 1 is more enriched than isoform 2 in developing brain as well as non-neuronal cells. Isoform 2 is very abundant in nerve terminals.
Similarity:
Belongs to the synaptojanin family.
In the central section; belongs to the inositol-1,4,5-trisphosphate 5-phosphatase family.
Contains 1 RRM (RNA recognition motif) domain.
Contains 1 SAC domain.
Database links:
Entrez Gene: 104015 Mouse
Entrez Gene: 85238 Rat
SwissProt: Q8CHC4 Mouse
SwissProt: Q62910 Rat
Unigene: 187079 Mouse
Unigene: 379876 Mouse
Unigene: 22685 Rat
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
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