Rabbit Anti-AP36/FITC Conjugated antibody

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AP 180; AP3; Assembly Protein 180 kDa; Assembly Protein AP180; CALM; Clathrin coat assembly protein; Clathrin coat assembly protein AP180; Clathrin coat associated protein AP180; DKFZp781O051; F120; KIAA0656; NP185; pp155; SNAP 91; SNAP91; 91kDa synaptoso

Cat:

SL11333R-FITC

Species Reactivity：

(predicted: Human,Mouse,Rat,Chicken,Dog,Pig,Cow,Horse,Sheep,)

Immunogen：

KLH conjugated synthetic peptide derived from human AP180

Format：

Lyophilized or Liquid

Storage instructions：

Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of ant

Buffer：

0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

ICC=1:50-200IF=1:50-200not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Calculated MW：

92kDa

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Unit:

Price: $596.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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background:
Clathrin-coated pits and vesicles are assembled for receptor-mediated endocytosis through interaction with Clathrin associated protein complexes. Vesicle transport is mediated from the trans-Golgi network by the adapter complex AP-1 and from the plasma membrane by the AP-2 complex. The AP-1 and AP-2 adapter protein complexes consist of Clathrin binding Adaptin proteins (g and b1 for AP-1, a and b2 for AP-2) and two smaller subunits known as AP50 and AP17. The a and b Adaptin chains have a similar two-domain organization with SLCterminal domains that vary in both sequence and length. a-Adaptin splice variants A and C display variable relative expression levels and differential distribution in different tissues. AP-3 (also designated AP36 or F1-20) is a synapse-specific Clathrin assembly protein. The protein CALM (Clathrin assembly protein lymphoid myeloid leukemia) is highly homologous to AP36 and may also be involved in Clathrin assembly.

Function:
AP36 is a clathrin binding phospho-protein and faciliates the formation of clathrin coats. This protein is also known as pp155, NP185, F1-20, and AP-3. During neurotransmitter release synaptic vesicles fuse with the presynaptic plasma membrane. A whole protein machinery consisting of amphiphysin, clathrin, endophilin and synaptojanin is involved in the subsequent endocytotic recycling of the synaptic vesicles.

Subunit:
Binds AP2A2. Interacts with AP2B1; clathrin competes with SNAP91 (By similarity).

Subcellular Location:
Component of the coat surrounding the cytoplasmic face of coated vesicles in the plasma membrane.

Post-translational modifications:
Thr-310 can be modified by the addition of N-acetylglucosamine which can be further phosphorylated. There is no evidence for direct Thr-310 phosphorylation (By similarity).

Similarity:
Contains 1 ENTH (epsin N-terminal homology) domain.

Database links:

Entrez Gene: 9892 Human

Entrez Gene: 20616 Mouse

Entrez Gene: 65178 Rat

Omim: 607923 Human

SwissProt: O60641 Human

SwissProt: Q61548 Mouse

SwissProt: Q05140 Rat

Unigene: 73646 Human

Unigene: 281651 Mouse

Unigene: 11022 Rat

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

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