Rabbit Anti-phospho-CTBP1 (Ser422)/FITC Conjugated antibody

Home > Product > Antibody > Rabbit Anti-phospho-CTBP1 (Ser422)/FITC Conjugated antibody

CTBP1 (phospho Ser422); p-CTBP1 (Ser422); CtBP1 (phospho S422); BARS; C terminal binding protein 1; CTBP; CTBP1; MGC104684; CTBP1_HUMAN; C-terminal-binding protein 1; CtBP1.

Cat:

SL11275R-FITC

Species Reactivity：

(predicted: Human,Mouse,Rat,Chicken,Dog,Cow,Horse,Danio rerio)

Immunogen：

KLH conjugated synthesised phosphopeptide derived from human CTBP1 around the phosphorylation site of Ser422

Format：

Lyophilized or Liquid

Storage instructions：

Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of ant

Buffer：

0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

ICC=1:50-200IF=1:50-200not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Calculated MW：

49kDa

prev next

Unit:

Price: $596.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
Feedback Wall

background:
CtBP1 associates in complexes with histone deacetylases (HDAC), the G9a histone methyltransferase, and LSD1 histone demethylase illustrating its role in transcriptional repression. Through its association with centrosomes, CtBP1 appears to also play a role in mitosis and Golgi complex organization.

Function:
Involved in controlling the equilibrium between tubular and stacked structures in the Golgi complex. Functions in brown adipose tissue (BAT) differentiation. Corepressor targeting diverse transcription regulators such as GLIS2. Has dehydrogenase activity.

Subunit:
Homo- or heterodimer. Heterodimer with CTBP2. Interacts with PRDM16; the interaction represses white adipose tissue (WAT)-specific genes expression. Interacts with GLIS2, FOXP2, HDAC4, HDAC5, HDAC9 and ZNF217 (By similarity). Interacts with adenovirus E1A protein (via its SLCterminus); the interaction disrupts the interaction of CTBP1 with RBBP8. Interacts with Epstein-Barr virus EBNA3 and EBNA6. Interacts with ELK3 (via its PXDLS motif). Interacts with RBBP8 (via its PXDLS motif); the interaction is disrupted by binding to adenovirus E1A. Interacts with FOXP1, HIPK2, PNN, NRIP1, MECOM, ZNF366, ZFHX1B and WIZ. Interaction with SATB1 (non-acetylated form); the interaction stabilizes its attachment to DNA and promotes transcription repression.

Subcellular Location:
Cytoplasm. Nucleus.

Post-translational modifications:
The level of phosphorylation appears to be regulated during the cell cycle. Phosphorylation by HIPK2 on Ser-422 induces proteasomal degradation.
ADP-ribosylated; when cells are exposed to brefeldin A.
Sumoylation on Lys-428 is promoted by the E3 SUMO-protein ligase CBX4.

Similarity:
Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family.

Database links:

Entrez Gene: 1487 Human

Entrez Gene: 13016 Mouse

Entrez Gene: 29382 Rat

Omim: 602618 Human

SwissProt: Q13363 Human

SwissProt: O88712 Mouse

SwissProt: Q9Z2F5 Rat

Unigene: 208597 Human

Unigene: 594321 Human

Unigene: 7286 Mouse

Unigene: 3946 Rat

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

Product Feedback Wall