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Rabbit Anti-Cdc25C/FITC Conjugated antibody
background:
This gene is highly conserved during evolution and it plays a key role in the regulation of cell division. The encoded protein is a tyrosine phosphatase and belongs to the Cdc25 phosphatase family. It directs dephosphorylation of cyclin B-bound CDC2 and triggers entry into mitosis. It is also thought to suppress p53-induced growth arrest. Multiple alternatively spliced transcript variants of this gene have been described, however, the full-length nature of many of them is not known. [provided by RefSeq, Jul 2008]
Function:
Functions as a dosage-dependent inducer in mitotic control. It is a tyrosine protein phosphatase required for progression of the cell cycle. It directly dephosphorylates CDK1 and activate its kinase activity.
Subunit:
Interacts with HISLV1 Vpr, thereby inactivating CDC25C phosphatase activity. Interacts with MAPK14 and 14-3-3 proteins.
Subcellular Location:
Nucleus.
Post-translational modifications:
Phosphorylated by CHEK1 and MAPK14 at Ser-216. This phosphorylation creates a binding site for 14-3-3 protein and inhibits the phosphatase. Phosphorylated by PLK4. Phosphorylated by PLK1, leading to activate the phosphatase activity. Phosphorylation by PLK3 at Ser-191 promotes nuclear translocation. Ser-198 is a minor phosphorylation site. Was initially reported to be phosphorylated by PLK3 at Ser-216 (PubMed:10557092). However, such phosphorylation by PLK3 was not confirmed by other groups. Phosphorylation at Thr-48, Thr-67, Ser-122, Thr-130, Ser-168 and Ser-214 occurs at G2 and G2-M transition and is probably catalyzed by CDK1. Ser-168 phosphorylation levels are lower than those at the other 5 CDK1 sites. Phosphorylation by CDK1 leads to increased activity.
Similarity:
Belongs to the MPI phosphatase family.
Contains 1 rhodanese domain.
Database links:
Entrez Gene: 995 Human
Omim: 157136 Human
SwissProt: P30307 Human
Unigene: 656 Human
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
CDC25家族的组成哺乳动物中CDC25家族一共包括3种同源异构体(CDC25A、CDC25B、CDC25C),约有50%的序列同源,是一组在细胞周期调控中发挥巨大作用的苏/酪氨酸双功能酶 。不同的CDC25家族蛋白在细胞周期中的作用时相亦有差异,CDC25A和CDC25C分别在S期和M期发挥主要作用。
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