background:
Protein trafficking to the membrane requires formation of coated carrier vesicles, such as COPI-coated vesicles from the cis-golgi, a process triggered by membrane binding of the GTP-bound form of ADP-ribosylation factors. Normally, brefeldin A (SFA) blocks this action by inhibiting guanine nucleotide exchange factors (GEFs) for ADP-ribosylation factor. However, GSF1, a member of the sec7-domain family of GEFs, allows cells to maintain normal golgi morphology and grow in the presence of SFA. The gene encoding the human GSF1 protein maps to chromosome 10q24, with the sec7-domain centrally positioned, and a proline-rich SLCterminus. Based on mutatagenesis analysis, this proline rich region appears to interact with p115 in a functionally significant manner.
Function:
Promotes guanine-nucleotide exchange on ARF5. Promotes the activation of ARF5 through replacement of GDP with GTP.
Tissue Specificity:
Ubiquitous.
Similarity:
Contains 1 SEC7 domain.
Database links:
Entrez Gene: 8729 Human
Entrez Gene: 107338 Mouse
Omim: 603698 Human
SwissProt: Q92538 Human
Unigene: 290243 Human
Unigene: 271620 Mouse
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
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