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Rabbit Anti-Musashi 1/FITC Conjugated antibody
background:
Musashi1 (Msi1) is an RNA-binding protein expressed in neural progenitor cells and neural stem cells. Msi1 is the mammalian homolog of Drosophila Musashi. The gene encoding human Msi1 encodes a 362 amino acid protein. In murine embryonic neural progenitor cells, Msi1 localizes to the cytoplasm and is downregulated during differentiation. Msi1 binds to NUMB, which encodes a membrane-associated antagonist of Notch signaling. Msi1 appears to function in the proliferation and maintenance of stem cell populations of the central nervous system. In addition to its usefulness as a marker for neural progenitor cells in normal human brains, Msi1 is also a marker for human gliomas. In rats, Msi1 is expressed in Sertoli cells of the testis and granulosa cells of the ovary.
Function:
RNA binding protein that regulates the expression of target mRNAs at the translation level. Regulates expression of the NOTCH1 antagonist NUMB. Binds RNA containing the sequence 5'-GUUAGUUAGUUAGUU-3' and other sequences containing the pattern 5'-[GA]U(1-3)AGU-3'. May play a role in the proliferation and maintenance of stem cells in the central nervous system.
Subcellular Location:
Cytoplasm. Nucleus.
Tissue Specificity:
Detected in fetal kidney, brain, liver and lung, and in adult brain and pancreas. Detected in hepatoma cell lines.
Similarity:
Belongs to the Musashi family.
Contains 2 RRM (RNA recognition motif) domains.
Database links:
Entrez Gene: 488 Human
Entrez Gene: 17690 Mouse
Entrez Gene: 259272 Rat
Omim: 603328 Human
SwissProt: O43347 Human
SwissProt: Q61474 Mouse
SwissProt: Q8K3P4 Rat
Unigene: 158311 Human
Unigene: 5077 Mouse
Unigene: 162146 Rat
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
Musashi1是一种进化保守的RNA结合蛋白，在维持干细胞状态、分化和肿瘤发生方面起着重要作用。Musashi1 选择性地表达在神经前体细胞上,包括神经干细胞上。在神经系统外，Musashi1还是肠干细胞的选择性标记。这些组织干细胞或未成熟细胞Musashi1的表达，表明Musashi1在转录后基因调节阶段维持这些细胞未分化状态起重要作用。Musashi1过度表达通过依赖RBP2Jk的旁路激活Notch1，而Notch信号途径功能为诱导哺乳动物神经干细胞自我更新。通过musashi1-P-小鼠培养脑细胞的Musashi蛋白产物反义去除研究,发现这些基因在维持神经干细胞未分化状态起着重要的作用。 Musashi抑制m-Numb转录的分子机制尚待进一步研究。Musashi1有可能除转录调控外还参与其他调控途径。另外，Musashi1还表达在一些脑肿瘤的特殊类型(这些肿瘤可能起源自非成熟脑细胞)，并且表达水平和肿瘤的恶性程度及增殖能力相关。
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