Rabbit Anti-KIF5A/NKHC1/FITC Conjugated antibody

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Kinesin 5A; Kinesin5A; Kinesin-5A; KIF 5A; KIF5A; KIF5A_HUMAN; Kinesin family member 5A; Kinesin heavy chain isoform 5A; Kinesin Heavy Chain Neuron Specific; Kinesin heavy chain neuron-specific 1; neuronal kinesin heavy chain 1; MY050; Neuronal kinesin he

Cat:

SL11027R-FITC

Species Reactivity：

(predicted: Human,Mouse,Rat,Chicken,Dog,Pig,Cow,Horse,Rabbit,Sheep,)

Immunogen：

KLH conjugated synthetic peptide derived from human KIF5A/NK

Format：

Lyophilized or Liquid

Storage instructions：

Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of ant

Buffer：

0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

ICC=1:50-200IF=1:50-200not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Calculated MW：

113kDa

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Unit:

Price: $596.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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background:
NKHC1 is a neuronal-specific component of a multi-subunit “molecular motor” complex that mediates intracellular organelle transport. Mutations in the gene encoding NKHC1 cause autosomal dominant spastic paraplegia 10. NKHC1 has a pan-neuronal distribution in the nervous system. Rat tissue extracts by immunoblot of NKHC1 can produce a doublet only in brain and sciatic nerve tissue. NKHC1 is distributed throughout the central nervous system and is enriched in subsets of neurons. Within cultured hippocampal neurons, NKHC1 is concentrated in the perinuclear region of the cell body. Kinesin superfamily proteins like NKHC1 are the molecular motors conveying cargos along microtubules.

Function:
Microtubule-dependent motor required for slow axonal transport of neurofilament proteins (NFH, NFM and NFL).

Subunit:
Oligomer composed of two heavy chains and two light chains. Interacts with GRIP1.

Subcellular Location:
Cytoplasm, perinuclear region. Cytoplasm, cytoskeleton. Note=Concentrated in the cell body of the neurons, particularly in the perinuclear region.

Tissue Specificity:
Distributed throughout the CNS but is highly enriched in subsets of neurons.

DISEASE:
Defects in KIF5A are the cause of spastic paraplegia autosomal dominant type 10 (SPG10). An inherited degenerative spinal cord disorder characterized by a slow, gradual, progressive weakness and spasticity (stiffness) of the legs. Rate of progression and the severity of symptoms is quite variable. Initial symptoms may include difficulty with balance, weakness and stiffness in the legs, muscle spasms, and dragging the toes when walking. In some forms of the disorder, bladder symptoms (such as incontinence) may appear, or the weakness and stiffness may spread to other parts of the body.

Similarity:
Belongs to the kinesin-like protein family. Kinesin subfamily.
Contains 1 kinesin-motor domain.

Database links:

Entrez Gene: 511273 Cow

Entrez Gene: 3798 Human

Entrez Gene: 16572 Mouse

Entrez Gene: 314906 Rat

Omim: 602821 Human

SwissProt: Q12168 Human

SwissProt: P33175 Mouse

SwissProt: Q6QLM7 Rat

Unigene: 151219 Human

Unigene: 30355 Mouse

Unigene: 63144 Rat

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

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