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Rabbit Anti-SMARCD3/FITC Conjugated antibody
background:
SMARCD3, is a member of the SMARCD family and contains one SWIB domain. Two isoforms, isoform 1 and isoform 2 exist due to alternative splicing events. Both isoforms are expressed in placenta, salivary gland, kidney, brain, trachea, uterus, prostate, testis, thyroid, spleen and heart, while isoform 1 is also expressed in adipose tissue and skeletal muscle. Localizing to the nucleus, SMARCD3 is a component of the ATP-dependent chromatin remodeling complex SNF/SWI and is believed to play a role in nucleosome remodeling. SMARCD3 also plays an important role in the regulation of muscle development. In mice, the silencing of the gene en-coding SMARCD3 leads to defects in heart morphogenesis. In addition, both isoforms of SMARCD3 directly interact with and function as coactivators for several transcription factors.
Function:
Plays a role in ATP dependent nucleosome remodeling bySMARCA4 containing complexes. Stimulates nuclear receptor mediatedtranscription. Belongs to the neural progenitors-specific chromatinremodeling complex (npBAF complex) and the neuron-specificchromatin remodeling complex (nBAF complex). During neuraldevelopment a switch from a stem/progenitor to a post-mitoticchromatin remodeling mechanism occurs as neurons exit the cellcycle and become committed to their adult state. The transitionfrom proliferating neural stem/progenitor cells to post-mitoticneurons requires a switch in subunit composition of the npBAF andnBAF complexes. As neural progenitors exit mitosis anddifferentiate into neurons, npBAF complexes which containACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologousalternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunitsin neuron-specific complexes (nBAF). The npBAF complex is essentialfor the self-renewal/proliferative capacity of the multipotentneural stem cells. The nBAF complex along with CREST plays a roleregulating the activity of genes essential for dendrite growth (Bysimilarity).
Subunit:
Component of the BAF complex, which includes at leastactin (ACTB), ARID1A, ARID1B/BAF250, SMARCA2, SMARCA4/BRG1/BAF190A,ACTL6A/BAF53, ACTL6B/BAF53B, SMARCE1/BAF57, SMARCC1/BAF155,SMARCC2/BAF170, SMARCB1/SNF5/INI1, and one or more ofSMARCD1/BAF60A, SMARCD2/BAF60B, or SMARCD3/BAF60C. In muscle cells,the BAF complex also contains DPF3. Interacts withSMARCA4/BRG1/BAF190A, the catalytic subunit of the SWI/SNF relatednucleosome-remodeling complexes BRG1(I) and BRG1(II). The precisedistribution of the related SMARCD1, SMARCD2 and SMARCD3 proteinsamong these and other SWI/SNF nucleosome-remodeling complexes isnot fully known. Also interacts with several nuclear receptorsincluding PPARG/NR1C3, RXRA/NR1F1, ESR1, NR5A1, NR5A2/LRH1 andother transcriptional activators including the HLH proteinSRESF1/SREBP1 and the homeobox protein PBX1. May allow recruitmentof Swi/SNF containing complexes specifically to promoters wherethese factors are located. Component of neural progenitors-specificchromatin remodeling complex (npBAF complex) composed of at least,ARID1A/BAF250A or ARID1B/BAF250B, SMARCD1/BAF60A, SMARCD3/BAF60C,SMARCA2/BRM/BAF190B, SMARCA4/BRG1/BAF190A, SMARCB1/BAF47,SMARCC1/BAF155, SMARCE1/BAF57, SMARCC2/BAF170, PHF10/BAF45A,ACTL6A/BAF53A and actin. Component of neuron-specific chromatinremodeling complex (nBAF complex) composed of at least,ARID1A/BAF250A or ARID1B/BAF250B, SMARCD1/BAF60A, SMARCD3/BAF60C,SMARCA2/BRM/BAF190B, SMARCA4/BRG1/BAF190A, SMARCB1/BAF47,SMARCC1/BAF155, SMARCE1/BAF57, SMARCC2/BAF170, DPF1/BAF45B,DPF3/BAF45C, ACTL6B/BAF53B and actin (By similarity).
Subcellular Location:
Nucleus.
Tissue Specificity:
Isoform 2 and isoform 1 are expressed in brain, heart, kidney, placenta, prostate, salivary gland, spleen, testis, thyroid, trachea and uterus. Isoform 1 is also expressed in skeletal muscle and adipose tissue.
Similarity:
Belongs to the SMARCD family.
Contains 1 SWIB domain.
Database links:
Entrez Gene: 6604 Human
Entrez Gene: 66993 Mouse
Entrez Gene: 296732 Rat
Omim: 601737 Human
SwissProt: Q6STE5 Human
SwissProt: Q6P9Z1 Mouse
Unigene: 647067 Human
Unigene: 436539 Mouse
Unigene: 20043 Rat
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
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