Rabbit Anti-Bone Sialoprotein/FITC Conjugated antibody

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BNSP; Bone sialoprotein II; BSP; BSPII; Cell binding sialoprotein; Integrin binding sialoprotein; SPII; SIAL_HUMAN; Bone sialoprotein 2; BSP II; Cell-binding sialoprotein; Integrin-binding sialoprotein.

Cat:

SL4729R-FITC

Species Reactivity：

Human,Mouse,(predicted: Rat,Dog,Pig,Horse,Guinea Pig,)

Immunogen：

KLH conjugated synthetic peptide derived from mouse Bone Sialoprotein

Format：

Lyophilized or Liquid

Storage instructions：

Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of ant

Buffer：

0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Calculated MW：

35kDa

Product Overview：

Blank control:MG63. Primary Antibody (green line): Rabbit Anti- Bone Sialoprotein antibody (SL4729R-FITC) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG .ProtocolThe cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20,000 events was performed.

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Unit:

Price: $596.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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background:
The protein encoded by this gene is a major structural protein of the bone matrix. It constitutes approximately 12% of the noncollagenous proteins in human bone and is synthesized by skeletal-associated cell types, including hypertrophic chondrocytes, osteoblasts, osteocytes, and osteoclasts. The only extraskeletal site of its synthesis is the trophoblast. This protein binds to calcium and hydroxyapatite via its acidic amino acid clusters, and mediates cell attachment through an RGD sequence that recognizes the vitronectin receptor. [provided by RefSeq, Jul 2008]

Function:
Binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction. Promotes Arg-Gly-Asp-dependent cell attachment.

Subcellular Location:
Secreted.

Post-translational modifications:
N-glycosylated; glycans consist of sialylated and core-fucosylated bi-, tri- and tetraantennary chains.
Sulfated on either Tyr-64 or Tyr-321 (By similarity).

Database links:

Entrez Gene: 3381 Human

Entrez Gene: 15891 Mouse

Entrez Gene: 24477 Rat

Omim: 147563 Human

SwissProt: P21815 Human

SwissProt: Q61711 Mouse

SwissProt: P13839 Rat

Unigene: 518726 Human

Unigene: 4987 Mouse

Unigene: 9721 Rat

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. Picture

Blank control:MG63.
Primary Antibody (green line): Rabbit Anti- Bone Sialoprotein antibody (SL4729R-FITC)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20,000 events was performed.

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