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Rabbit Anti-FEN1/FITC Conjugated antibody
background:
Structure-specific nuclease with 5'-flap endonuclease and 5'-3' exonuclease activities involved in DNA replication and repair. During DNA replication, cleaves the 5'-overhanging flap structure that is generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment. It enters the flap from the 5'-end and then tracks to cleave the flap base, leaving a nick for ligation. Also involved in the long patch base excision repair (LP-BER) pathway, by cleaving within the apurinic/apyrimidinic (AP) site-terminated flap. Acts as a genome stabilization factor that prevents flaps from equilibrating into structurs that lead to duplications and deletions. Also possesses 5'-3' exonuclease activity on nicked or gapped double-stranded DNA, and exhibits RNase H activity. Also involved in replication and repair of rDNA and in repairing mitochondrial DNA.
Subunit:
Interacts with PCNA. Three molecules of FEN1 bind to one PCNA trimer with each molecule binding to one PCNA monomer. PCNA stimulates the nuclease activity without altering cleavage specificity. The SLCterminal domain binds EP300. Can bind simultaneously to both PCNA and EP300. Interacts with DDX11.
Subcellular Location:
Nucleus, nucleolus. Nucleus, nucleoplasm. Mitochondrion. Note=Resides mostly in the nucleoli and relocalizes to the nucleoplasm upon DNA damage.
Similarity:
Belongs to the XPG/RAD2 endonuclease family. FEN1 subfamily.
Database links:
Entrez Gene: 2237 Human
Entrez Gene: 14156 Mouse
Omim: 600393 Human
SwissProt: P39748 Human
SwissProt: P39749 Mouse
Unigene: 409065 Human
Unigene: 2952 Mouse
Unigene: 470595 Mouse
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
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