Rabbit Anti-RNF61/MKRN1/FITC Conjugated antibody

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RING finger protein 61; E3 ubiquitin-protein ligase makorin-1; Makorin; Makorin ring finger protein 1; MKRN 1; MKRN-1; mkrn1; MKRN1_HUMAN; Ring finger protein 1; RNF61.

Cat:

SL9378R-FITC

Species Reactivity：

(predicted: Human,Mouse,Rat,Dog,Pig,Cow,Horse,Rabbit,Sheep,)

Immunogen：

KLH conjugated synthetic peptide derived from human MKRN1/RNF61

Format：

Lyophilized or Liquid

Storage instructions：

Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of ant

Buffer：

0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

IF=1:50-200not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Calculated MW：

53kDa

Product Overview：

Blank control: A431. Primary Antibody (green line): Rabbit Anti-RNF61/MKRN1 antibody (SL9378R-FITC) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . ProtocolThe cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20,000 events was performed.

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Unit:

Price: $596.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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background:
E3 ubiquitin ligase catalyzing the covalent attachment of ubiquitin moieties onto substrate proteins. These substrates include FILIP1, p53/TP53, CDKN1A and TERT. Keeps cells alive by suppressing p53/TP53 under normal conditions, but stimulates apoptosis by repressing CDKN1A under stress conditions. Acts as a negative regulator of telomerase. Has negative and positive effects on RNA polymerase II-dependent transcription.

Function:
E3 ubiquitin ligase catalyzing the covalent attachment of ubiquitin moieties onto substrate proteins. These substrates include FILIP1, p53/TP53, CDKN1A and TERT. Keeps cells alive by suppressing p53/TP53 under normal conditions, but stimulates apoptosis by repressing CDKN1A under stress conditions. Acts as a negative regulator of telomerase. Has negative and positive effects on RNA polymerase II-dependent transcription.

Subunit:
Interacts with p53/TP53 and CDKN1A. Interacts with TERT, modulating telomere length homeostasis.

Tissue Specificity:
Ubiquitous.

Post-translational modifications:
Auto-ubiquitinated; which leads to proteasomal degradation.

Similarity:
Contains 4 C3H1-type zinc fingers.
Contains 1 RING-type zinc finger.

Database links:

Entrez Gene: 2728 Human

Entrez Gene: 54484 Mouse

Entrez Gene: 296988 Rat

Omim: 607754 Human

SwissProt: Q9UHC7 Human

SwissProt: Q9QXP6 Mouse

Unigene: 728819 Human

Unigene: 270484 Mouse

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. Picture

Blank control: A431.
Primary Antibody (green line): Rabbit Anti-RNF61/MKRN1 antibody (SL9378R-FITC)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Protocol
The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20,000 events was performed.

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