Rabbit Anti-MARCH5/FITC Conjugated antibody

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E3 ubiquitin-protein ligase MARCH5; MARCH-V; Membrane-associated RING finger protein 5; Membrane-associated RING-CH protein V; RING finger protein 153.

Cat:

SL9339R-FITC

Species Reactivity：

(predicted: Human,Mouse,Rat,Chicken,Dog,Cow,)

Immunogen：

KLH conjugated synthetic peptide derived from human MARCH5

Format：

Lyophilized or Liquid

Storage instructions：

Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of ant

Buffer：

0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

IF=1:50-200not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Calculated MW：

31kDa

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Unit:

Price: $596.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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background:
MARCH5 is a member of the E3 ubiquitin-protein ligase family. This mitochonrial E3 ubiquitin-protein ligase plays an importnat role in controlling the morphology of the mitochondria. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfer the ubiquitin to targeted substrates. MARCH5 has been shown to interact with MTFN2 and ubiquitinated forms of DRP1.

Function:
Mitochondrial E3 ubiquitin-protein ligase that plays a crucial role in the control of mitochondrial morphology by acting as a positive regulator of mitochondrial fission. May play a role in the prevention of cell senescence acting as a regulator of mitochondrial quality control. Promotes ubiquitination of FIS1, DNM1L and MFN1.

Subunit:
Monomer and homodimer. Interacts with MFN1, MFN2, DNM1L and FIS1.

Subcellular Location:
Mitochondrion outer membrane; Multi-pass membrane protein. Endoplasmic reticulum membrane. Note=Authors show that the protein can be detected in endoplasmic reticulum (PubMed:14722266). Authors (PubMed:16874301) show its presence only in mitochondria (PubMed:16874301).

Tissue Specificity:
Expressed in brain, heart, liver, lung, spleen, stomach, testis, skeletal and muscle.

Post-translational modifications:
Autoubiquitinated leading to degradation (short half-life).

Similarity:
Contains 1 RING-CH-type zinc finger.

Database links:

Entrez Gene: 54708 Human

Entrez Gene: 69104 Mouse

Entrez Gene: 294079 Rat

Omim: 610637 Human

SwissProt: Q9NX47 Human

Unigene: 573490 Human

Unigene: 175989 Mouse

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

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