Rabbit Anti-RNF123/FITC Conjugated antibody

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E3 ubiquitin protein ligase RNF123; Kip1 ubiquitination promoting complex protein 1; KPC 1; KPC1; Ring finger protein 123; RNF 123; Ubiquitin ligase;

Cat:

SL9249R-FITC

Species Reactivity：

Mouse,(predicted: Human,Rat,Dog,Pig,)

Immunogen：

KLH conjugated synthetic peptide derived from human RNF123

Format：

Lyophilized or Liquid

Storage instructions：

Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of ant

Buffer：

0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

IF=1:50-200not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Calculated MW：

149kDa

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Unit:

Price: $596.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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background:
The RING-type zinc finger motif is present in a number of viral and eukaryotic proteins and is made of a conserved cysteine-rich domain that is able to bind two zinc atoms. Proteins that contain this conserved domain are generally involved in protein-protein interactions and protein-DNA interactions. RNF123 (RING finger protein 123), also known as KPC1 (Kip1 (p27) ubiquitination-promoting complex protein 1) or FP1477, contains one RING-type zinc finger domain and one SPRY domain. Localizing to the cytoplasm, RNF123 functions as the catalytic component of the KPC complex that acts as an E3 ubiquitin-protein ligase. Specifically, RNF123 is essential for the ubiquitination and subsequent degradation of p27 during the cell cycle G1 phase. Via its N-terminus, RNF123 is known to interact with GBDR1 (another component of the KPC) and p27 (a cyclin-dependent kinase inhibitor). Due to alternative splicing events, two isoforms exist for RNF123.

Function:
Catalytic subunit of the KPC complex that acts as E3 ubiquitin-protein ligase. Required for poly-ubiquitination and proteasome-mediated degradation of CDKN1B during G1 phase of the cell cycle.

Subunit:
Component of the KPC complex composed of RNF123/KPC1 and UBAC1/KPC2. Interacts with UBAC1 and CDKN1B via its N-terminal domain.

Subcellular Location:
Cytoplasmic

Post-translational modifications:
Ubiquitinated, leading to its degradation. Deubiquitinated by USP19, thereby stimulating CDKN1B ubiquitin-dependent degradation (By similarity).

Similarity:
Contains 1 B30.2/SPRY domain.
Contains 1 RING-type zinc finger.

Database links:

Entrez Gene: 63891 Human

SwissProt: Q5XPI4 Human

Unigene: 553723 Human

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

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