background:
The ubiquitin (Ub) pathway involves three sequential enzymatic steps that facilitate the conjugation of Ub and Ub-like molecules to specific protein substrates. Through the use of a wide range of enzymes that can add or remove ubiquitin, the Ub pathway controls many intracellular processes such as signal transduction, transcriptional activation and cell cycle progression. USP3 (ubiquitin specific peptidase 3), also known as UBP or SIH003, is a 520 amino acid protein that contains one UBP-type zinc finger and belongs to the peptidase C19 family. Expressed ubiquitously with highest levels present in pancreas, USP3 catalyzes the conversion of a ubiquitin SLCterminal thioester to a free ubiquitin and a thiol. The gene encoding USP3 maps to human chromosome 15, which houses over 700 genes and comprises nearly 3% of the human genome.
Function:
Hydrolase that deubiquitinates monoubiquitinated target proteins such as histone H2A and H2B. Required for proper progression through S phase and subsequent mitotic entry. May regulate the DNA damage response (DDR) checkpoint through deubiquitination of H2A at DNA damage sites. Associates with the chromatin.
Subunit:
Interacts (via UBP-type domain) with H2A; the interaction is less efficient than with monoubiquitinated H2A.
Subcellular Location:
Nucleus. Localizes preferentially with monoubiquitinated H2A to chromatin.
Tissue Specificity:
Expressed in all tissues examined, with strongest expression in pancreas.
Similarity:
Belongs to the peptidase C19 family. USP3 subfamily.
Contains 1 UBP-type zinc finger.
Database links:
UniProtKB/Swiss-Prot: Q9Y6I4.2
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
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