Home > Product > Antibody > Rabbit Anti-IQCD/FITC Conjugated antibody
IQ domain containing protein D; IQ domain-containing protein D; IQ motif containing D; IQCD; IQCD_HUMAN.
Cat:
SL9017R-FITC
Species Reactivity:
Human,(predicted: Mouse,Rat,Cow,Horse,Sheep,)
Immunogen:
KLH conjugated synthetic peptide derived from human IQCD
Format:
Lyophilized or Liquid
Storage instructions:
Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of ant
Buffer:
0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Concentration:
1mg/ml
Clonality:
Polyclonal
Isotype:
IgG
Applications:
Flow-Cyt=2ug/TestIF=1:50-200not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.
Host:
Rabbit
Calculated MW:
52kDa
Product Overview:
Blank control: A549. Primary Antibody (green line): Rabbit Anti-IQCD antibody (SL9017R-FITC) Dilution: 2μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . ProtocolThe cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1%PBST for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20,000 events was performed.
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Unit:
Price: $
Product PDFs
Datasheet:


Similarity:
Contains 1 IQ domain.

Database links:

Entrez Gene: 115811 Human

Entrez Gene: 75732 Mouse

SwissProt: Q96DY2 Human

SwissProt: Q9D3V1 Mouse

SwissProt: Q5XIR6 Rat

Unigene: 568276 Human

Unigene: 425285 Mouse

Unigene: 137813 Rat



Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. Picture

Blank control: A549.
Primary Antibody (green line): Rabbit Anti-IQCD antibody (SL9017R-FITC)
Dilution: 2μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1%PBST for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20,000 events was performed.
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