Similarity:
Contains 1 IQ domain.
Database links:
Entrez Gene: 115811 Human
Entrez Gene: 75732 Mouse
SwissProt: Q96DY2 Human
SwissProt: Q9D3V1 Mouse
SwissProt: Q5XIR6 Rat
Unigene: 568276 Human
Unigene: 425285 Mouse
Unigene: 137813 Rat
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
Picture |
Blank control: A549.
Primary Antibody (green line): Rabbit Anti-IQCD antibody (SL9017R-FITC)
Dilution: 2μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1%PBST for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20,000 events was performed.
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