Rabbit Anti-CD71/Transferrin receptor/FITC Conjugated antibody

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CD 71; CD71; CD71 antigen; p90; T9; TFR 1; TFR; TFR1; TFRC; TR; Transferrin receptor (p90 CD71); Transferrin Receptor Protein 1; OTTHUMP00000208523; OTTHUMP00000208524; OTTHUMP00000208525; Transferrin receptor protein 1, serum form; TRFR.

Cat:

SL0988R-FITC

Species Reactivity：

Human,Mouse,(predicted: Rat,Dog,Pig,Cow,Horse,Guinea Pig,)

Immunogen：

KLH conjugated synthetic peptide derived from human Transferrin receptor

Format：

Lyophilized or Liquid

Storage instructions：

Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of ant

Buffer：

0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

Flow-Cyt=2ug/Testnot yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Calculated MW：

74/85kDa

Product Overview：

Blank control: Molt4. Primary Antibody (green line): Rabbit Anti-CD71 antibody (SL0988R-FITC) Dilution: 2μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . ProtocolThe cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20,000 events was performed.

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Unit:

Price: $596.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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background:
Cellular uptake of iron occurs via receptor mediated endocytosis of ligand occupied transferrin receptor into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system. Useful in studies of dividing haematopoietic and tumour cell populations, and metabolic activity. A second ligand, the heditary hemochromatosis protein HFE, competes for binding with transferrin for an overlapping C terminal binding site. The antigen is present on most dividing cells, including normally cycling in vivo hematopoietic progenitor cells, mitogenically stimulated cells in vitro, some primary tumor cells and most proliferating cells in vitro.

Function:
Cellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied transferrin receptor into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system. A second ligand, the heditary hemochromatosis protein HFE, competes for binding with transferrin for an overlapping SLCterminal binding site.

Subunit:
Homodimer; disulfide-linked. Binds one transferrin molecule per subunit. Interacts with SH3BP4.

Subcellular Location:
Secreted and Cell membrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.

Post-translational modifications:
N- and O-glycosylated, phosphorylated and palmitoylated. The serum form is only glycosylated. Proteolytically cleaved on Arg-100 to produce the soluble serum form (sTfR). Palmitoylated on both Cys-62 and Cys-67. Cys-62 seems to be the major site of palmitoylation.

Similarity:
Belongs to the peptidase M28 family. M28B subfamily.
Contains 1 PA (protease associated) domain.

Database links:

Entrez Gene: 7037 Human

Entrez Gene: 22042 Mouse

Entrez Gene: 64678 Rat

Omim: 190010 Human

SwissProt: P02786 Human

SwissProt: Q62351 Mouse

SwissProt: Q99376 Rat

Unigene: 529618 Human

Unigene: 28683 Mouse

Unigene: 98672 Rat

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

转铁蛋白及转铁蛋白受体(TfnrR)是机体铁代谢中的重要因子，铁是细胞生长增殖、代谢的必需元素,转铁蛋白受体(TfR)介导的胞吞作用是细胞摄取铁的主要途径。肿瘤细胞因增殖生长旺盛,对铁的需求高,所以肿瘤细胞有比正常细胞更多的转铁蛋白受体的表达。 Picture

Blank control: Molt4.
Primary Antibody (green line): Rabbit Anti-CD71 antibody (SL0988R-FITC)
Dilution: 2μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Protocol
The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20,000 events was performed.

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