Rabbit Anti-phospho-MYF5 (phospho Ser49)/FITC Conjugated antibody

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Myf5 (phospho S49); Myf5 (phospho Ser49); p-Myf5(phospho S49); p-Myf5(phospho Ser49); Myf-5 bHLHc2; Class C basic helix loop helix protein 2; Class C basic helix-loop-helix protein 2; Myf 5; Myf-5; Myf5; MYF5_HUMAN; Myogenic factor 5.

Cat:

SL8200R-FITC

Species Reactivity：

(predicted: Human,Mouse,Horse,)

Immunogen：

KLH conjugated synthesised phosphopeptide derived from human MYF5 around the phosphorylation site of Ser49

Format：

Lyophilized or Liquid

Storage instructions：

Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of ant

Buffer：

0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

IF=1:50-200not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Calculated MW：

28kDa

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Unit:

Price: $596.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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background:
Differentiation of myogenic cells is regulated by multiple positively and negatively acting factors. One well characterized family of helix-loop-helix (HLH) proteins known to play an important role in the regulation of muscle cell development include Myo D, myogenin, Myf-5 and Myf-6 (also designated MRF-4 or herculin). Of interest, most muscle cells express either Myo D or Myf-5 in the committed state, but when induced to differentiate, all turn on expression of myogenin. Myo D transcription factors form heterodimers with products of a more widely expressed family of bHLH genes, the E family, which consists of at least three distinct genes: E2A, IF2 and HEB. Myo D-E heterodimers bind avidly to consensus (CANNTG) E box target sites that are functionally important elements in the upstream regulatory sequences of many muscle-specific terminal differentiation genes.

Function:
Involved in muscle differentiation (myogenic factor). Induces fibroblasts to differentiate into myoblasts. Probable sequence specific DNA-binding protein.

Subunit:
Efficient DNA binding requires dimerization with another bHLH protein.

Subcellular Location:
Nucleus.

Similarity:
Contains 1 bHLH (basic helix-loop-helix) domain.

Database links:

Entrez Gene: 4617 Human

Entrez Gene: 17877 Mouse

Entrez Gene: 299766 Rat

Omim: 159990 Human

SwissProt: P13349 Human

SwissProt: P24699 Mouse

Unigene: 35623 Human

Unigene: 4984 Mouse

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

yf5是MyoD基因家族的一员，该家族的共同结构特点是具有1个保守的中央蛋白基序(motif)，大小为80个氨基酸，称为碱性螺旋-环-螺旋(bHLH)，bHLH高度保守，其中Basic结构是HLH螺旋结构的延伸，也是这一家族蛋白与DNA相互作用的区域，而HLH螺旋结构则是与其他因子相互作用的位点，即调控的重要区域。
生肌决定因子(MyoD)基因家族控制着肌细胞的增殖和分化，与肌纤维的数量、大小有着密切的关系，因而对肉质和风味有非常重要的作用，MyoD基因家族可编码4种不同的转录因子，分别为MyoD(Myf3)、Myogenin(MyoG)、Myf5、Myf6或MRF4，它们各自或协同控制着骨骼肌生成方面的关键调节因子。

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