Rabbit Anti-KIRREL1/FITC Conjugated antibody

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Kin of IRRE like (Drosophila); Kin of IRRE like; Kin of IRRE like protein 1; Kin of IRRE-like protein 1; Kin of irregular chiasm like protein 1; Kin of irregular chiasm-like protein 1; KIRR1_HUMAN; Kirrel; MGC129542; MGC129543; NEPH 1; NEPH1; Nephrin like

Cat:

SL6435R-FITC

Species Reactivity：

(predicted: Human,Mouse,Rat,)

Immunogen：

KLH conjugated synthetic peptide derived from human KIRREL1/NEPH1

Format：

Lyophilized or Liquid

Storage instructions：

Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of ant

Buffer：

0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

IF=1:50-200not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Calculated MW：

82kDa

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Unit:

Price: $596.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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background:
Neph1(Kirrel) belongs to a family of three closely related transmembrane proteins of the Ig superfamily with a structure similar to that of nephrin. Neph1 consists of a signal peptide, five Ig-like C2-type domains with the middle domain overlapping with a PKD-like domain, an RGD sequence, a transmembrane domain and a cytoplasmic tail. Neph1 is abundantly expressed in the kidney, specifically expressed in podocytes of kidney glomeruli, and plays a significant role in the normal development and function of the glomerular permeability. Neph1 interacts with nephrin in vitro and in vivo, and able to stimulate transcriptional activation in a model system, such as the activation the transcription factor AP-1 via the stimulation of a MAPK module. Neph1 is crucial for the integrity of the slit diaphragm, as Neph1 gene knockout mice results in effacement of glomerular podocytes, heavy proteinuria, and early postnatal death. .
Tissue specificity:
Abundantly expressed in kidney. Specifically expressed in podocytes of kidney glomeruli.

Function:
Plays a significant role in the normal development and function of the glomerular permeability. Signaling protein that needs the presence of TEC kinases to fully trans-activate the transcription factor AP-1 (By similarity).

Subunit:
Interacts with TJP1/ZO-1 and with NPHS2/podocin (via the SLCterminus). Interacts with NPHS1/nephrin (via the Ig-like domains); this interaction is dependent on KIRREL glycosylation. Homodimer (via the Ig-like domains). Interacts when tyrosine-phosphorylated with GRB2 (By similarity).

Subcellular Location:
Cell membrane; Single-pass type I membrane protein.

Tissue Specificity:
Abundantly expressed in kidney. Specifically expressed in podocytes of kidney glomeruli.

Post-translational modifications:
Phosphorylation probably regulates the interaction with NSH2. Phosphorylated at Tyr-605 and Tyr-606 by FYN, leading to GRB2 binding (By similarity).
N-glycosylated (By similarity).

Similarity:
Belongs to the immunoglobulin superfamily.
Contains 5 Ig-like C2-type (immunoglobulin-like) domains.

Database links:
UniProtKB/Swiss-Prot: Q96J84.2

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

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