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Rabbit Anti-HCF-1/FITC Conjugated antibody
background:
This gene is a member of the host cell factor family and encodes a protein with five Kelch repeats, a fibronectin-like motif, and six HCF repeats, each of which contains a highly specific cleavage signal. This nuclear coactivator is proteolytically cleaved at one of the six possible sites, resulting in the creation of an N-terminal chain and the corresponding SLCterminal chain. The final form of this protein consists of noncovalently bound N- and SLCterminal chains. The protein is involved in control of the cell cycle and transcriptional regulation during herpes simplex virus infection. Alternatively spliced variants which encode different protein isoforms have been described; however, not all variants have been fully characterized. [provided by RefSeq, Jul 2008]
Function:
Involved in control of the cell cycle. Also antagonizestransactivation by ZBTB17 and GABP2; represses ZBTB17 activation ofthe p15(INK4b) promoter and inhibits its ability to recruit p300.Coactivator for EGR2 and GABP2. Tethers the chromatin modifyingSet1/Ash2 histone H3 'Lys-4' methyltransferase (H3K4me) and Sin3histone deacetylase (HDAC) complexes (involved in the activationand repression of transcription, respectively) together. Componentof a THAP1/THAP3-HCFC1-OGT complex that is required for theregulation of the transcriptional activity of RRM1. As part of theNSL complex it may be involved in acetylation of nucleosomalhistone H4 on several lysine residues. In case of human herpessimplex virus (HSV) infection, HCFC1 forms a multiprotein-DNAcomplex with the viral transactivator protein VP16 and POU2F1thereby enabling the transcription of the viral immediate earlygenes.
Subunit:
Composed predominantly of six polypeptides ranging from110 to 150 kDa and a minor 300 kDa polypeptide. The majority ofN-and SLCterminal cleavage products remain tightly, albeitnon-covalently, associated. Interacts with POU2F1, CREB3, ZBTB17,EGR2, E2F4, CREBZF, SP1, GABP2, Sin3 HDAC complex (SIN3A, HDAC1,HDAC2, SDS3), SAP30, SIN3B and FHL2. Component of a MLL1 complex,composed of at least the core components MLL, ASH2L, HCFC1, WDR5and RBBP5, as well as the facultative components C17orf49, CHD8,DPY30, E2F6, HCFC2, HSP70, INO80C, KANSL1, LAS1L, MAX, MCRS1, MEN1,MGA, KAT8, PELP1, PHF20,. PRP31, RING2, RUVBL1, RUVBL2, SENP3,TAF1, TAF4, TAF6, TAF7, TAF9 and TEX10. Component of the MLL5-Lcomplex, composed of at least MLL5, STK38, PPP1CA, PPP1CB, PPP1CC,HCFC1, ACTB and OGT. Component of a THAP1/THAP3-HCFC1-OGT complexthat is required for the regulation of the transcriptional activityof RRM1. Interacts directly with OGT; the interaction, whichrequires the HCFC1 cleavage site domain, glycosylates and promotesthe proteolytic processing of HCFC1, retains OGT in the nucleus andimpacts the expression of herpes simplex virus immediate earlyviral genes. Interacts directly with THAP3 (via its HBM). Interacts(via the Kelch-repeat domain) with THAP1 (via the HBM); theinteraction recruits HCHC1 to the RRM1. Interacts with HCFC1R1 andTHAP11. Associates with the VP16-induced complex; binding to HCFC1activates the viral transcriptional activator VP16 for associationwith POU2F1, to form a multiprotein-DNA complex responsible foractivating transcription of the viral immediate early genes.Component of the SET1 complex, at least composed of the catalyticsubunit (SETD1A or SETD1B), WDR5, WDR82, RBBP5, ASH2L, CXXC1, HCFC1and DPY30. Component of the NSL complex at least composed ofMOF/KAT8, KANSL1, KANSL2, KANSL3, MCRS1, PHF20, OGT1/OGT, WDR5 andHCFC1.
Subcellular Location:
Cytoplasm. Nucleus. Note=HCFC1R1 modulates its subcellular localization and overexpression of HCFC1R1 leads to accumulation of HCFC1 in the cytoplasm. Nuclear in general, but uniquely cytoplasmic in trigeminal ganglia, becoming nuclear upon HSV reactivation from the latent state. Non-processed HCFC1 associates with chromatin.
Tissue Specificity:
Highly expressed in fetal tissues and the adult kidney. Present in all tissues tested.
Post-translational modifications:
Proteolytically cleaved at one or several PPCE--THET siteswithin the HCF repeats. Further cleavage of the primary N- andSLCterminal chains results in a 'trimming' and accumulation of thesmaller chains. Cleavage is promoted by O-glycosylation.
O-glycosylated. O-glycosylation promotes proteolyticprocessing.
Ubiquitinated. Lys-367 and Lys-368 are ubiquitinated bothvia 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains. BAP1mediated deubiquitination of 'Lys-48'-linked polyubiquitin chains;deubiquitination by BAP1 does not seem to stabilize the protein.
Similarity:
Contains 5 Kelch repeats.
Database links:
Entrez Gene: 3054 Human
Entrez Gene: 15161 Mouse
Omim: 300019 Human
SwissProt: P51610 Human
SwissProt: Q61191 Mouse
Unigene: 83634 Human
Unigene: 248353 Mouse
Unigene: 439140 Mouse
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
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