background:
Involved in intracellular protein transport probably out of a prevacuolar endosomal compartment. May be involved in the release of components of the bilayered coat from the endosomal membrane. The association with ESCRT-III complex mediates the ATP-dependent disassembly of the ESCRT-III complex. In case of infection, the HISLV1 virus takes advantage of it for budding and exocytic cargos of viral proteins.
Function:
Involved in late steps of the endosomal multivesicular bodies (MVB) pathway. Recognizes membrane-associated ESCRT-III assemblies and catalyzes their disassembly, possibly in combination with membrane fission. Redistributes the ESCRT-III components to the cytoplasm for further rounds of MVB sorting. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. In conjunction with the ESCRT machinery also appears to function in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis and enveloped virus budding (HISLV1 and other lentiviruses). Involved in cytokinesis.
Subunit:
Proposed to be monomeric or homodimeric in nucleotide-free form and to oligomerize upon binding to ATP to form two stacked hexameric or heptameric rings with a central pore through which ESCRT-III substrates are translocated in an ATP-dependent manner (By similarity). Interacts with CHMP1A, CHMP1B, CHMP2A, CHMP2B, CHMP3, CHMP4A, CHMP4B, CHMP4C and CHMP6. Interacts with VPS4B; the interaction suggests a heteromeric assembly with VPS4B. Interacts with SPAST. Interacts with IST1.
Subcellular Location:
Prevacuolar compartment membrane; Peripheral membrane protein. Late endosome membrane; Peripheral membrane protein (Probable). Note=Membrane-associated in the prevacuolar endosomal compartment. Localizes to the midbody of dividing cells. Localized in two distinct rings on either side of the Fleming body.
Tissue Specificity:
Ubiquitously expressed.
Similarity:
Belongs to the AAA ATPase family.
Contains 1 MIT domain.
Database links:
Entrez Gene: 27183 Human
Entrez Gene: 116733 Mouse
Entrez Gene: 246772 Rat
Omim: 609982 Human
SwissProt: Q9UN37 Human
SwissProt: Q8VEJ9 Mouse
SwissProt: Q793F9 Rat
Unigene: 128420 Human
Unigene: 27204 Mouse
Unigene: 12477 Rat
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
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