Rabbit Anti-DFFB/FITC Conjugated antibody

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CAD; Caldesmon; Caspase activated deoxyribonuclease; Caspase activated DNase; Caspase activated nuclease; CDM; CPAN; Deoxyribonuclease III, caspase activated; DFF 40; DFF2; DFF40; Didff; DNA fragmentation factor 40 kDa subunit; DNA fragmentation factor su

Cat:

SL7648R-FITC

Species Reactivity：

Mouse,(predicted: Human,Rat,Dog,Rabbit,)

Immunogen：

KLH conjugated synthetic peptide derived from human DFFB

Format：

Lyophilized or Liquid

Storage instructions：

Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of ant

Buffer：

0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

IF=1:50-200not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Calculated MW：

39kDa

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Unit:

Price: $596.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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background:
Apoptosis is a cell death process that removes toxic and/or useless cells during mammalian development. The apoptotic process is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. DNA fragmentation factor (DFF) is a heterodimeric protein of 40-kD (DFFB) and 45-kD (DFFA) subunits. DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF becomes activated when DFFA is cleaved by caspase-3. The cleaved fragments of DFFA dissociate from DFFB, the active component of DFF. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene but the biological validity of these variants has not been determined. [provided by RefSeq, Jul 2008].

Function:
Nuclease that induces DNA fragmentation and chromatin condensation during apoptosis. Degrades naked DNA and induces apoptotic morphology.

Subunit:
Heterodimer of DFFA and DFFB. Interacts with HIST1H1A.

Subcellular Location:
Cytoplasm. Nucleus.

Similarity:
Contains 1 CIDE-N domain.

Database links:

Entrez Gene: 1677 Human

Entrez Gene: 13368 Mouse

Entrez Gene: 84359 Rat

Omim: 601883 Human

SwissProt: O76075 Human

SwissProt: O54788 Mouse

SwissProt: Q99N34 Rat

Unigene: 133089 Human

Unigene: 388918 Mouse

Unigene: 67077 Rat

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

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