Rabbit Anti-EZH1/FITC Conjugated antibody

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Enhancer of zeste homolog 1(Drosophila); Enhancer of zeste homolog 1; ENX-2; ENX2; EZH1; EZH1: enhancer of zeste homolog 1 (Drosophila); EZH1_HUMAN; Histone-lysine N-methyltransferase EZH1; KIAA0388.

Cat:

SL6528R-FITC

Species Reactivity：

Human,Mouse,Rat,(predicted: Chicken,Dog,Pig,Cow,Rabbit,Guinea Pig,)

Immunogen：

KLH conjugated synthetic peptide derived from human EZH1

Format：

Lyophilized or Liquid

Storage instructions：

Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of ant

Buffer：

0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

IF=1:50-200not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Calculated MW：

85kDa

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Unit:

Price: $596.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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background:
Polycomb group (PcG) protein. Catalytic subunit of the PRC2/EED-EZH1 complex, which methylates 'Lys-27' of histone H3, leading to transcriptional repression of the affected target gene. Able to mono-, di- and trimethylate 'Lys-27' of histone H3 to form H3K27me1, H3K27me2 and H3K27me3, respectively. Required for embryonic stem cell derivation and self-renewal, suggesting that it is involved in safeguarding embryonic stem cell identity. Compared to EZH1-containing complexes, it is less abundant in embryonic stem cells, has weak methyltransferase activity and plays a less critical role in forming H3K27me3, which is required for embryonic stem cell identity and proper differentiation.
Sequence similarities
Belongs to the histone-lysine methyltransferase family. EZ subfamily. Contains 1 SET domain.

Function:
Polycomb group (PcG) protein. Catalytic subunit of the PRC2/EED-EZH1 complex, which methylates 'Lys-27' of histone H3, leading to transcriptional repression of the affected target gene. Able to mono-, di- and trimethylate 'Lys-27' of histone H3 to form H3K27me1, H3K27me2 and H3K27me3, respectively. Required for embryonic stem cell derivation and self-renewal, suggesting that it is involved in safeguarding embryonic stem cell identity. Compared to EZH1-containing complexes, it is less abundant in embryonic stem cells, has weak methyltransferase activity and plays a less critical role in forming H3K27me3, which is required for embryonic stem cell identity and proper differentiation.

Subunit:
Component of the PRC2/EED-EZH1 complex, which includes EED, EZH1, SUZ12, RBBP4 and AEBP2. The PRC2/EED-EZH1 is less abundant than the PRC2/EED-EZH2 complex, has weak methyltransferase activity and compacts chromatin in the absence of the methyltransferase cofactor S-adenosyl-L-methionine (SAM).

Subcellular Location:
Nucleus. Note=Colocalizes with trimethylated 'Lys-27' of histone H3.

Similarity:
Belongs to the histone-lysine methyltransferase family. EZ subfamily.
Contains 1 CXC domain.
Contains 1 SET domain.

Database links:

Entrez Gene: 2145 Human

Entrez Gene: 14055 Mouse

Entrez Gene: 303547 Rat

Omim: 601674 Human

SwissProt: Q92800 Human

SwissProt: P70351 Mouse

Unigene: 194669 Human

Unigene: 5027 Mouse

Unigene: 154549 Rat

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

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