Rabbit Anti-DOK3/FITC Conjugated antibody

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Docking protein 3; DOK 3; DOK Like Protein; DOKL Pending; Downstream of tyrosine kinase 3; p62 DOK Like Protein; DOK3_HUMAN.

Cat:

SL6234R-FITC

Species Reactivity：

Mouse,(predicted: Human,Rat,Cow,)

Immunogen：

KLH conjugated synthetic peptide derived from human DOK3

Format：

Lyophilized or Liquid

Storage instructions：

Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of ant

Buffer：

0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

IF=1:50-200not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Calculated MW：

53kDa

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Unit:

Price: $596.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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background:
DOK proteins are enzymatically inert adaptor or scaffolding proteins. They provide a docking platform for the assembly of multimolecular signaling complexes. DOK3 is a negative regulator of JNK signaling in B-cells through interaction with INPP5D/SHIP1. May modulate Abl functionDOK proteins are enzymatically inert adaptor or scaffolding proteins. They provide a docking platform for the assembly of multimolecular signaling complexes. DOK3 is a negative regulator of JNK signaling in B-cells through interaction with INPP5D/SHIP1. May modulate Abl function. There are 4 isoforms generated by alternative splicing.

Function:
DOK proteins are enzymatically inert adaptor or scaffolding proteins. They provide a docking platform for the assembly of multimolecular signaling complexes. DOK3 is a negative regulator of JNK signaling in B-cells through interaction with

Subunit:
On tyrosine phosphorylation, interacts with CSK and INPP5D/SHIP1 via their SH2 domains. Both Tyr-381 and Tyr-398 are required for interaction with INPP5D. Only Tyr-381 is required for interaction with CSK. Binds ABL1 through the PTB domain and in a kinase-dependent manner. Does not interact with RasGAP (By similarity).

Subcellular Location:
Cytoplasm (By similarity). Cell membrane; Peripheral membrane protein; Cytoplasmic side (By similarity).

Tissue Specificity:
Expressed in spleen.

Post-translational modifications:
Constitutively tyrosine-phosphorylated (By similarity).
On IL2 stimulation, phosphorylated on SLCterminal tyrosine residues possibly by Src kinases. Can also be phosphorylated by ABL1 kinase (By similarity).

Similarity:
Belongs to the DOK family. Type A subfamily.
Contains 1 IRS-type PTB domain.
Contains 1 PH domain.

Database links:

Entrez Gene: 79930 Human

Entrez Gene: 27261 Mouse

Entrez Gene: 306760 Rat

Omim: 611435 Human

SwissProt: Q7L591 Human

SwissProt: Q9QZK7 Mouse

SwissProt: B2RYG7 Rat

Unigene: 720849 Human

Unigene: 33910 Mouse

Unigene: 19911 Rat

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

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