Rabbit Anti-HACE1/FITC Conjugated antibody

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E3 ubiquitin-protein ligase HACE1; HACE 1; HECT domain and ankyrin repeat-containing E3 ubiquitin-protein ligase 1; KIAA1320.

Cat:

SL6011R-FITC

Species Reactivity：

(predicted: Human,Mouse,Rat,Chicken,Dog,Pig,Cow,Horse,)

Immunogen：

KLH conjugated synthetic peptide derived from human HACE1

Format：

Lyophilized or Liquid

Storage instructions：

Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of ant

Buffer：

0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

IF=1:50-200not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Calculated MW：

90-102kDa

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Unit:

Price: $596.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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background:
E3 ubiquitin-protein ligase that may function in cellular proteins degradation. HACE1 has also been shown to be down-regulated in many human tumours and is a putative candidate tumor suppressor.

Function:
E3 ubiquitin-protein ligase involved in Golgi membrane fusion and regulation of small GTPases. Acts as a regulator of Golgi membrane dynamics during the cell cycle: recruited to Golgi membrane by Rab proteins and regulates postmitotic Golgi membrane fusion. Acts by mediating ubiquitination during mitotic Golgi disassembly, ubiquitination serving as a signal for Golgi reassembly later, after cell division. Specifically interacts with GTP-bound RAC1, mediating ubiquitination and subsequent degradation of active RAC1, thereby playing a role in host defense against pathogens. May also act as a transcription regulator via its interaction with RARB.

Subunit:
Interacts with RARB (By similarity). Interacts with RAB1 (RAB1A, RAB1B or RAB1C), RAB4 (RAB4A or RAB4B) and RAB11 (RAB11A or RAB11B); in a GTP-dependent manner. Interacts with RAC1; in a GTP-dependent manner. Interacts with the 26S proteasomal complex through the 20S core proteasomal subunit.

Subcellular Location:
Cytoplasm. Endoplasmic reticulum. A significant portion localizes to the endoplasmic reticulum.

Tissue Specificity:
Expressed in multiple tissues including heart, brain and kidney.

DISEASE:
Note=Defects in HACE1 are a cause of Wilms tumor (WT). WT is a pediatric malignancy of kidney and one of the most common solid cancers in childhood. HACE1 is epigenetically down-regulated in sporadic Wilms tumor. Moreover, a t(5;6)(q21;q21) translocation that truncates HACE1 has been found in a child with bilateral, young-onset Wilms tumor.

Similarity:
Contains 6 ANK repeats.
Contains 1 HECT (E6AP-type E3 ubiquitin-protein ligase) domain.

Database links:

Entrez Gene: 527565 Cow

Entrez Gene: 100733867 Guinea pig

Entrez Gene: 100071887 Horse

Entrez Gene: 57531 Human

Entrez Gene: 209462 Mouse

Entrez Gene: 100156102 Pig

Entrez Gene: 361866 Rat

Omim: 610876 Human

SwissProt: F1N6G5 Cow

SwissProt: Q8IYU2 Human

SwissProt: Q3U0D9 Mouse

SwissProt: D3ZBM7 Rat

Unigene: 434340 Human

Unigene: 458633 Mouse

Unigene: 28116 Rat

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

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