Rabbit Anti-SMYD3/FITC Conjugated antibody

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SET and MYND domain containing 3; SET and MYND domain containing protein 3; SMYD 3; SMYD3 protein; Zinc finger MYND domain containing 1; Zinc finger MYND domain containing protein 1; Zinc finger protein subfamily 3A MYND domain containing 1; ZMYND 1; ZMYN

Cat:

SL5974R-FITC

Species Reactivity：

Human,(predicted: Mouse,Rat,Cow,Horse,Rabbit,Sheep,)

Immunogen：

KLH conjugated synthetic peptide derived from human SMYD3

Format：

Lyophilized or Liquid

Storage instructions：

Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of ant

Buffer：

0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

IF=1:50-200not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Calculated MW：

49kDa

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Unit:

Price: $596.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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background:
SMYD3 is a gene that is over-expressed in the majority of colorectal carcinomas and hepatocellular carcinomas. SMYD3 forms a complex with RNA polymerase II through an interaction with the RNA helicase HELZ and transactivates a set of genes that included oncogenes, homeobox genes and genes associated with cell-cycle regulation. SMYD3 binds to a motif, 5'-CCCTCSLC3', present in the promoter region of downstream genes such as Nkx2.8. The SET domain of SMYD3 shows histone H3-lysine 4 (H3-K4)-specific methyltransferase activity, which is enhanced in the presence of the heat-shock protein HSP90A.

Function:
Histone methyltransferase. Specifically methylates 'Lys-4' of histone H3, inducing di- and tri-methylation, but not monomethylation. Plays an important role in transcriptional activation as a member of an RNA polymerase complex. Binds DNA containing 5'-CCCTCSLC3' or 5'-GAGGGG-3' sequences.

Subunit:
Interacts with HSPCA. Interacts with HELZ. Interacts with POLR2A; the interaction may be indirect and may be mediated by HELZ.

Subcellular Location:
Cytoplasm. Nucleus. Note=Mainly cytoplasmic when cells are arrested at G0/G1. Accumulates in the nucleus at S phase and G2/M.

Tissue Specificity:
Expressed in skeletal muscles and testis. Overexpressed in a majority of colorectal and hepatocellular carcinomas.

Similarity:
Belongs to the histone-lysine methyltransferase family.
Contains 1 MYND-type zinc finger.
Contains 1 SET domain.

Database links:

Entrez Gene: 64754 Human

Entrez Gene: 69726 Mouse

Entrez Gene: 498295 Rat

Omim: 608783 Human

SwissProt: Q9H7B4 Human

SwissProt: Q9CWR2 Mouse

Unigene: 567571 Human

Unigene: 222338 Mouse

Unigene: 28284 Rat

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

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