background:
Molecular calcium-binding chaperone promoting folding, oligomeric assembly and quality control in the ER via the calreticulin/calnexin cycle. This lectin interacts transiently with almost all of the monoglucosylated glycoproteins that are synthesized in the ER. Interacts with the DNA-binding domain of NR3C1 and mediates its nuclear export.
Subunit:
Monomer. Component of an EIF2 complex at least composed of CELF1/CUGBP1, CALR, CALR3, EIF2S1, EIF2S2, HSP90B1 and HSPA5. Interacts with PDIA3/ERp57 (By similarity). Interacts with NR3C1 and TRIM21. Interacts with GABARAP.
Subcellular Location:
Endoplasmic reticulum lumen. Cytoplasm, cytosol. Secreted, extracellular space, extracellular matrix. Cell surface.
Similarity:
Belongs to the calreticulin family.
Database links:
Entrez Gene: 41166 Fruit fly (Drosophila melanogaster)
Entrez Gene: 811 Human
Entrez Gene: 12317 Mouse
Entrez Gene: 100009050 Rabbit
Entrez Gene: 64202 Rat
Omim: 109091 Human
SwissProt: P29413 Fruit fly (Drosophila melanogaster)
SwissProt: P27797 Human
SwissProt: P14211 Mouse
SwissProt: P15253 Rabbit
SwissProt: P18418 Rat
Unigene: 2457 Fruit fly (Drosophila melanogaster)
Unigene: 515162 Human
Unigene: 1971 Mouse
Unigene: 467043 Mouse
Unigene: 974 Rat
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
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Blank control:K562.
Primary Antibody (green line): Rabbit Anti-Calreticulin antibody (SL5913R)
Dilution: 1ug/Test;
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at -20℃.The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature.Acquisition of 20,000 events was performed.
Blank control:K562.
Primary Antibody (green line): Rabbit Anti-Calreticulin antibody (SL5913R-FITC)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature.Acquisition of 20,000 events was performed.
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