Rabbit Anti-MCPIP1/FITC Conjugated antibody

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dJ423B22.1; MCP 1 treatment-induced protein; MCP-induced protein 1; MCPIP 1; MCPIP; Ribonuclease ZC3H12A; RP3-423B22.1; ZC12A_HUMAN; ZC3H 12A; ZC3H12 A; Zc3h12a; Zinc finger CCCH domain-containing protein 12A; Zinc finger CCCH type containing 12A.

Cat:

SL5792R-FITC

Species Reactivity：

Human,Mouse,(predicted: Rat,Chicken,Dog,Cow,Horse,Rabbit,)

Immunogen：

KLH conjugated synthetic peptide derived from human MCPIP1

Format：

Lyophilized or Liquid

Storage instructions：

Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of ant

Buffer：

0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

Flow-Cyt=1ug/TestIF=1:50-200not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Calculated MW：

66kDa

Product Overview：

Blank control:Mouse spleen. Primary Antibody (green line): Rabbit Anti-MCPIP1 antibody (SL5792R-FITC) Dilution: 2μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . ProtocolThe cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20,000 events was performed.Blank control (Black line): HeLa (Black). Primary Antibody (green line): Rabbit MCPIP1 antibody (SL5792R) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Dilution: 3μg /test. ProtocolThe cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature.Acquisition of 20,000 events was performed.Blank control: Hela. Primary Antibody (green line): Rabbit Anti-MCPIP1 antibody (SL5792R-FITC) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . ProtocolThe cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at -20℃. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature.Acquisition of 20,000 events was performed.

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Unit:

Price: $596.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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background:
Has RNase activity and selectively degrades specific target mRNA species. Modulates the immune response and inflammation by regulating the decay of specific mRNA molecules. Recognizes the 3'-untranslated region (UTR) of the mRNA for IL6, CALCR and IL12B. Required for normal decay of IL6 mRNA (By similarity). Triggers apoptosis and promotes angiogenesis in response to the binding of CCL2 to CCR2. Regulates expression of CDH12 and CHD19.

Function:
Has RNase activity and selectively degrades specific target mRNA species. Modulates the immune response and inflammation by regulating the decay of specific mRNA molecules. Recognizes the 3'-untranslated region (UTR) of the mRNA for IL6, CALCR and IL12B. Required for normal decay of IL6 mRNA (By similarity). Triggers apoptosis and promotes angiogenesis in response to the binding of CCL2 to CCR2. Regulates expression of CDH12 and CHD19.

Subcellular Location:
Cytoplasm. Nucleus.

Similarity:
Belongs to the ZC3H12 family.
Contains 1 C3H1-type zinc finger.

Database links:

Entrez Gene: 535344 Cow

Entrez Gene: 80149 Human

Entrez Gene: 230738 Mouse

Omim: 610562 Human

SwissProt: A6QQJ8 Cow

SwissProt: Q5D1E8 Human

SwissProt: Q5D1E7 Mouse

Unigene: 656294 Human

Unigene: 402 Mouse

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. Picture

Blank control (Black line): HeLa (Black).
Primary Antibody (green line): Rabbit MCPIP1 antibody (SL5792R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Dilution: 3μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature.Acquisition of 20,000 events was performed.

Blank control: Hela.
Primary Antibody (green line): Rabbit Anti-MCPIP1 antibody (SL5792R-FITC)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at -20℃. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature.Acquisition of 20,000 events was performed.

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