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Rabbit Anti-PRRSSLVGP5/FITC Conjugated antibody
background:
PRRSV is a small, enveloped RNA virus. It contains a single-stranded, positive-sense, RNA genome with a size of approximately 15 kilobases. The genome contains nine open reading frames. PRRSV is a member of the genus Arterivirus, family Arteriviridae, order Nidovirales. Subclinical infections are common, with clinical signs occurring sporadically in a herd. Clinical signs include reproductive failure in sows such as abortions and giving birth to stillborn or mummified fetuses, and cyanosis of the ear and vulva. In neonatal pigs, the disease causes respiratory distress, with increased susceptibility to respiratory infections such as Glasser's disease.
Function:
The replicase polyprotein 1ab is a multifunctional protein: it contains the activities necessary for the transcription of negative stranded RNA, leader RNA, subgenomic mRNAs and progeny virion RNA as well as proteinases responsible for the cleavage of the polyprotein into functional products.
Nsp1 is essential for viral subgenomic mRNA synthesis.
Nsp1-alpha inhibits IFN-beta production. Counteracts the action of NF-kappaB by decreasing the phosphorylation of IkappaB-alpha, such that the degradation of IkappaB-alpha is suppressed. This leads to the blockage of NF-kappaB nuclear translocation and thus interference of NF-kappaB activation. Also seems to inhibit IRF3-dependent pathways.
Nsp1-beta seems to inhibit the translocation of STAT1 into the cell nucleus.
Nsp2 cysteine proteinase which cleaves the nsp2/nsp3 site in the polyprotein. Also displays deubiquitinating and deISGylase activities. The deubiquitinating activity cleaves both ubiquitinated and ISGylated products and may therefore regulate ubiquitin and ISG15 dependent host innate immunity. Deubiquitinates host NFKBIA, thereby interfering with NFKBIA degradation and impairing subsequent NF-kappa-B activation.
The 3SLClike serine proteinase chain is responsible for the majority of cleavages as it cleaves the SLCterminus of the polyprotein.
The helicase chain, which contains a zinc finger structure, displays RNA and DNA duplex-unwinding activities with 5' to 3' polarity.
Post-translational modifications:
Specific enzymatic cleavages in vivo by its own proteases yield mature proteins. Nsp1 is autocleaved into two subunits, Nsp1-alpha and Nsp1-beta. There are two alternative pathways for processing. Either nsp4-5 is cleaved, which represents the major pathway or the nsp5-6 and nsp6-7 are processed, which represents the minor pathway. The major pathway occurs when nsp2 acts as cofactor for nsp4.
Similarity:
Belongs to the arteriviridae polyprotein family.
Contains 1 (+)RNA virus helicase ATP-binding domain.
Contains 1 (+)RNA virus helicase SLCterminal domain.
Contains 1 AV MBD (arterivirus metal-binding) domain.
Contains 1 peptidase C31 domain.
Contains 1 peptidase C32 domain.
Contains 1 peptidase C33 domain.
Contains 1 peptidase S32 domain.
Contains 1 RdRp catalytic domain.
Database links:
Entrez Gene: 1494885 PRRSR
SwissProt: Q9WJB2 PRRSR
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
猪繁殖与呼吸综合征(Porcine reproductive and respiratory syndrome，PRRS)也称猪蓝耳病，是由动脉炎病毒科动脉炎病毒属的猪繁殖与呼吸综合征病毒,(Porcine reproductive and respiratory syndrome virus，PRRSV)引起的一种严重的病毒性传染病，该病以引起母猪的繁殖障碍、仔猪和育成猪呼吸道症状及高死亡率为主要特征。自1987年首次发现以来，该病广泛流行于世界各主要养猪国家和地区，已成为威胁养猪业的主要疫病之一。2006年以来，由该病毒变异株引起的高致病性猪蓝耳病(Highlypathogenicporcinereproductiveandrespiratorysyndrome，HPPRRS)在我国广泛流行给养猪业带来了巨大的损失。
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