Rabbit Anti-Squalene Epoxidase/FITC Conjugated antibody

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ERG1; SE antibody SQLE; Squalene monooxygenase; ERG1_HUMAN.

Cat:

SL4218R-FITC

Species Reactivity：

Human,Rat,(predicted: Mouse,Chicken,Dog,Pig,Cow,Horse,Rabbit,)

Immunogen：

KLH conjugated synthetic peptide derived from human Squalene Epoxidase

Format：

Lyophilized or Liquid

Storage instructions：

Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of ant

Buffer：

0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

IF=1:50-200not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Calculated MW：

64kDa

Product Overview：

Blank control:A431. Primary Antibody (green line): Rabbit Anti-SQLE antibody (SL4218R-FITC) Dilution: 2μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . ProtocolThe cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20,000 events was performed.

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Unit:

Price: $596.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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background:
This protein catalyzes the first oxygenation step in sterol biosynthesis and is suggested to be one of the rate-limiting enzymes in this pathway.

Function:
Catalyzes the first oxygenation step in sterol biosynthesis and is suggested to be one of the rate-limiting enzymes in this pathway.

Subunit:
May form a complex with squalene synthase.

Subcellular Location:
Microsome membrane; Multi-pass membrane protein. Endoplasmic reticulum membrane; Multi-pass membrane protein.

Similarity:
Belongs to the squalene monooxygenase family.

Database links:

Entrez Gene: 6713 Human

Entrez Gene: 20775 Mouse

Entrez Gene: 29230 Rat

Omim: 602019 Human

SwissProt: Q14534 Human

SwissProt: P52019 Mouse

SwissProt: P52020 Rat

Unigene: 71465 Human

Unigene: 296169 Mouse

Unigene: 33239 Rat

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. Picture

Blank control:A431.
Primary Antibody (green line): Rabbit Anti-SQLE antibody (SL4218R-FITC)
Dilution: 2μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20,000 events was performed.

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