Rabbit Anti-Phospho-RASGRF1 (Ser929)/FITC Conjugated antibody

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RASGRF1(phospho S929); RASGRF1(phospho Ser929); p-RASGRF1(S929); p-RASGRF1(Ser929); CDC25; CDC25L; GNRP; GRF1; GRF55; Guanine nucleotide exchange factor; Guanine nucleotide releasing factor 55 kD; Guanine nucleotide releasing protein; HGRF55; PP13187; Ras

Cat:

SL5683R-FITC

Species Reactivity：

(predicted: Human,Mouse,Rat,Chicken,Dog,Pig,Cow,Sheep,)

Immunogen：

KLH conjugated Synthesised phosphopeptide derived from human RasGRF1 around the phosphorylation site of Ser929

Format：

Lyophilized or Liquid

Storage instructions：

Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of ant

Buffer：

0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

IF=1:50-200not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Calculated MW：

145kDa

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Unit:

Price: $596.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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background:
The protein encoded by this gene is a guanine nucleotide exchange factor (GEF) similar to the Saccharomyces cerevisiae CDC25 gene product. Functional analysis has demonstrated that this protein stimulates the dissociation of GDP from RAS protein. The studies of the similar gene in mouse suggested that the Ras-GEF activity of this protein in brain can be activated by Ca2+ influx, muscarinic receptors, and G protein beta-gamma subunit. Mouse studies also indicated that the Ras-GEF signaling pathway mediated by this protein may be important for long-term memory. Alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Mar 2009]

Function:
Promotes the exchange of Ras-bound GDP by GTP.

Subunit:
Homooligomer and heterooligomer with RASGRF2. Interacts with USP8, thereby regulating its stability.

Post-translational modifications:
Phosphorylated by PLK2, leading to ubiquitination and degradation by the proteasome.
Ubiquitinated and degraded following phosphorylation by PLK2 By similarity).
Phosphorylated by SRC and LCK. Phosphorylation by LCK increases its capacity to stimulate the GDP/GTP exchange on Ras, whereas its phosphorylation by SRC seems not to have an effect on stimulation activity.

Similarity:
Contains 1 DH (DBL-homology) domain.
Contains 1 IQ domain.
Contains 1 N-terminal Ras-GEF domain.
Contains 2 PH domains.
Contains 1 Ras-GEF domain.

Database links:
Entrez Gene: 5923 Human

Entrez Gene: 192213 Rat

Omim: 606600 Human

SwissProt: Q13972 Human

SwissProt: P28818 Rat

Unigene: 459035 Human

Unigene: 135776 Rat

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

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