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Rabbit Anti-Phospho-Wee1(Ser53)/FITC Conjugated antibody
background:
This gene encodes a nuclear protein, which is a tyrosine kinase belonging to the Ser/Thr family of protein kinases. This protein catalyzes the inhibitory tyrosine phosphorylation of CDC2/cyclin B kinase, and appears to coordinate the transition between DNA replication and mitosis by protecting the nucleus from cytoplasmically activated CDC2 kinase.
Function:
Acts as a negative regulator of entry into mitosis (G2 to M transition) by protecting the nucleus from cytoplasmically activated cyclin B1-complexed CDK1 before the onset of mitosis by mediating phosphorylation of CDK1 on 'Tyr-15'. Specifically phosphorylates and inactivates cyclin B1-complexed CDK1 reaching a maximum during G2 phase and a minimum as cells enter M phase. Phosphorylation of cyclin B1-CDK1 occurs exclusively on 'Tyr-15' and phosphorylation of monomeric CDK1 does not occur. Its activity increases during S and G2 phases and decreases at M phase when it is hyperphosphorylated. A correlated decrease in protein level occurs at M/G1 phase, probably due to its degradation.
Subcellular Location:
Nucleus.
Post-translational modifications:
Phosphorylated during M and G1 phases. Also autophosphorylated. Phosphorylation at Ser-642 by BRSK1 and BRSK2 in post-mitotic neurons, leads to down-regulate WEE1 activity in polarized neurons. Phosphorylated at Ser-53 and Ser-123 by PLK1 and CDK1, respectively, generating an signal for degradation that can be recognized by the SCF(BTRC) complex, leading to its ubiquitination and degradation at the onset of G2/M phase.
Ubiquitinated and degraded at the onset of G2/M phase.
Similarity:
Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. WEE1 subfamily.
Contains 1 protein kinase domain.
Database links:
Entrez Gene: 7465 Human
Entrez Gene: 22390 Mouse
Entrez Gene: 308937 Rat
Omim: 193525 Human
SwissProt: P30291 Human
SwissProt: P47810 Mouse
SwissProt: Q63802 Rat
Unigene: 249441 Human
Unigene: 287173 Mouse
Unigene: 208255 Rat
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
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