Rabbit Anti-Phospho-Paxillin (Ser83)/FITC Conjugated antibody

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PXN(phospho S83); Paxillin (Phospho Ser83); Paxillin (Phospho S83); Paired box protein Pax 1; PAX 1; PAX1; Paxillin alpha; PXN; PXN protein; FLJ16691; FLJ23042; FLJ16691; PAXI_HUMAN; Paxillin; PXN protein.

Cat:

SL5549R-FITC

Species Reactivity：

Mouse,(predicted: Rat,)

Immunogen：

KLH conjugated Synthesised phosphopeptide derived from rat Paxillin around the phosphorylation site of Ser83

Format：

Lyophilized or Liquid

Storage instructions：

Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of ant

Buffer：

0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

IF=1:50-200not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Calculated MW：

68kDa

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Unit:

Price: $596.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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background:
Pxn(phospho S83); Paxillin is a 64 kDa cytoskeletal adapter protein involved in organisation and function of focal adhesions, which are critical to cell adhesion and migration. This in turn plays a role in a wide variety of processes including embryogenesis, organogenesis, wound repair, inflammation and cancer. Paxillin contains LD motifs, LIM domains, SH3 and SH2 binding domains that serve as docking sites for cytoskeletal proteins, tyrosine kinases (e.g., FAK, Pyk 2, Src), serine/threonine kinases, GTPase activating proteins and other adaptor proteins (e.g., Actin, Vinculin, Crk).

Function:
Cytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion).

Subunit:
Binds in vitro to vinculin as well as to the SH3 domain of SRC and, when tyrosine phosphorylated, to the SH2 domain of SLVCRK. Isoform beta binds to PTK2/FAK1 but weakly to vinculin. Isoform gamma binds to vinculin but only weakly to PTK2/FAK1. Interacts with GIT1, NUDT16L1/SDOS, PARVA and TGFB1I1. Component of cytoplasmic complexes, which also contain GIT1, ARHGEF6 and PAK1. Interacts with PTK2/FAK1 and PTK2B/PYK2. Binds ASAP2. Interacts with unphosphorylated ITGA4. Interacts with RNF5 and PDCD10. Interacts with NEK3 and this interaction is prolactin-dependent. Interacts with PTK6.

Subcellular Location:
Cytoplasm, cytoskeleton. Cell junction, focal adhesion. Cytoplasm, cell cortex.

Post-translational modifications:
Phosphorylated by MAPK1/ERK2. Phosphorylated on tyrosine residues during integrin-mediated cell adhesion, embryonic development, fibroblast transformation and following stimulation of cells by mitogens. Phosphorylation at Ser-244 by CDK5 reduces its interaction with PTK2/FAK1 in matrix-cell focal adhesions (MCFA) during oligodendrocytes (OLs) differentiation. Phosphorylation at Tyr-31 and Tyr-118 by PTK6 promote the activation of RAC1 via CRK/CrKII, thereby promoting migration and invasion.

Similarity:
Belongs to the paxillin family.
Contains 4 LIM zinc-binding domains.

Database links:

Entrez Gene: 5829 Human

Entrez Gene: 19303 Mouse

Entrez Gene: 72820 Rat

Omim: 602505 Human

SwissProt: P49023 Human

SwissProt: Q8VI36 Mouse

SwissProt: Q66H76 Rat

Unigene: 446336 Human

Unigene: 18714 Mouse

Unigene: 75 Rat

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

Paxillin是一种分子量为68KDa的局部粘附蛋白，参与肿瘤细胞的粘附与转移。主要用于各种恶性肿瘤的研究。 Paxillin 蛋白是胞外基质细胞粘附（粘着斑）位点上肌动蛋白-膜附着的细胞骨架蛋白。 Paxillin 在整合蛋白的信号传导中起着非常重要的作用，整合蛋白介导的细胞骨架的再组织需要，paxillin 酪氨酸残基的磷酸化。 Paxillin 被粘着斑激酶 (FAK)在其118位的酪氨酸残基处磷酸化。已经证明，paxillin 118位和31位两个酪氨酸的磷酸化对于MM1癌细胞的迁移是重要的。

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