Rabbit Anti-phospho-MEK5(Ser311+Thr315)/FITC Conjugated antibody

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MEK5 (phospho S311 + T315); p-MEK5 (phospho S311 + T315); MAP2K5 (phospho S311/T315); Dual specificity mitogen activated protein kinase kinase 5; Dual specificity mitogen-activated protein kinase kinase 5; EC 2.7.12.2; HsT17454; MAP kinase kinase 5;

Cat:

SL5430R-FITC

Species Reactivity：

(predicted: Human,Mouse,Rat,Chicken,Dog,Pig,Cow,Horse,Rabbit,Sheep,)

Immunogen：

KLH conjugated Synthesised phosphopeptide derived from human MEK5 around the phosphorylation site of Ser311+Thr315

Format：

Lyophilized or Liquid

Storage instructions：

Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of ant

Buffer：

0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

IF=1:50-200not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Calculated MW：

49kDa

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Unit:

Price: $596.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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background:
MEK5 is a dual specificity protein kinase belonging to thr Ser/Thr protein kinase family, (MAP kinase kinase family). It is activated by phosphorylation on Ser/Thr by MAP kinase kinases and interacts specifically with ERK5, and not with another MAP kinase like P38. This kinase specifically interacts with and activates MAPK7/ERK5. This kinase itself can be phosphorylated and activated by MAP3K3/MEKK3, as well as by atypical protein kinase C isoforms (aPKCs). It is not phosphorylated by RAFA, RAFB or RAFC and it may interact with GTPases such as CDC42. The signal cascade mediated by this kinase is involved in growth factor stimulated cell proliferation and muscle cell differentiation. MEK5 is expressed in many adult tissues and is most abundant in heart and skeletal muscle.

Function:
Acts as a scaffold for the formation of a ternary MAP3K2/MAP3K3-MAP3K5-MAPK7 signaling complex. Activation of this pathway appear to play a critical role in protecting cells from stress-induced apopotosis, neuronal survival and cardiac development and angiogenesis.

Subunit:
Interacts with PARD6A, MAP3K3 and MAPK7. Forms a complex with SQSTM1 and PRKCZ or PRKCI (By similarity). Interacts with Yersinia yopJ.

Tissue Specificity:
Expressed in many adult tissues. Abundant in heart and skeletal muscle.

Post-translational modifications:
Activated by phosphorylation on Ser/Thr by MAP kinase kinase kinases.
Yersinia yopJ may acetylate Ser/Thr residues, preventing phosphorylation and activation, thus blocking the MAPK signaling pathway.

Similarity:
Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily.
Contains 1 OPR domain.
Contains 1 protein kinase domain.

Database links:

Entrez Gene: 5607 Human

Entrez Gene: 23938 Mouse

Entrez Gene: 29568 Rat

Omim: 602520 Human

SwissProt: Q13163 Human

SwissProt: Q9WVS7 Mouse

SwissProt: Q62862 Rat

Unigene: 114198 Human

Unigene: 325746 Mouse

Unigene: 11054 Rat

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

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