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Rabbit Anti-NEK6/FITC Conjugated antibody
background:
The protein encoded by this gene is a kinase required for progression through the metaphase portion of mitosis. Inhibition of the encoded protein can lead to apoptosis. This protein also can enhance tumorigenesis by suppressing tumor cell senescence. Several transcript variants encoding a few different isoforms have been found for this gene.
Function:
Protein kinase which plays an important role in mitotic cell cycle progression. Required for chromosome segregation at metaphase-anaphase transition, robust mitotic spindle formation and cytokinesis. Phosphorylates ATF4, CIR1, PTN, RAD26L, RBBP6, RPS7, RPS6KB1, TRIP4, STAT3 and histones H1 and H3. Phosphorylates KIF11 to promote mitotic spindle formation. Involved in G2/M phase cell cycle arrest induced by DNA damage. Inhibition of activity results in apoptosis. May contribute to tumorigenesis by suppressing p53/TP53-induced cancer cell senescence.
Subunit:
Interacts with STAT3 and RPS6KB1 (By similarity). Interacts with NEK9, predominantly in mitosis. Interacts with KIF11 (via SLCterminus). Interacts with APBB1 (via WW domain). Interacts with ANKRA2, ATF4, ARHGAP33, CDC42, CIR1, PRAM1, PTN, PRDX3, PIN1, RAD26L, RBBP6, RPS7 and TRIP4.
Subcellular Location:
Cytoplasm. Nucleus. Nucleus speckle. Cytoplasm, cytoskeleton, centrosome. Cytoplasm, cytoskeleton, spindle pole. Note=Co-localizes with APBB1 at the nuclear speckles. Co-localizes with PIN1 in the nucleus. Co-localizes with ATF4, CIR1, ARHGAP33, ANKRA2, CDC42, NEK9, RAD26L, RBBP6, RPS7, TRIP4, RELB and PHF1 in the centrosome. Localizes to spindle microtubules in metaphase and anaphase and to the midbody during cytokinesis. [ALTERNATIVE PRODUCTS] Event=Alternative splicing; Named isoforms=4; Name=1; IsoId=Q9HC98-1; Sequence=Displayed; Name=2; IsoId=Q9HC98-2; Sequence=VSP_041798; Name=3; IsoId=Q9HC98-3; Sequence=VSP_041799; Name=4; IsoId=Q9HC98-4; Sequence=VSP_04360.
Tissue Specificity:
Ubiquitous, with highest expression in heart and skeletal muscle. Up-regulated in a variety of malignant cancers, such as breast, colon, lung, and gastric cancers.
Post-translational modifications:
Autophosphorylated. Phosphorylation at Ser-206 is required for its activation. Phosphorylated upon IR or USLVinduced DNA damage. Phosphorylated by CHEK1 and CHEK2. Interaction with APBB1 down-regulates phosphorylation at Thr-210.
Similarity:
Belongs to the protein kinase superfamily. NEK Ser/Thr protein kinase family. NIMA subfamily.
Contains 1 protein kinase domain.
Database links:
Clone ID: BC004174 Human
Entrez Gene: 10783 Human
Entrez Gene: 59126 Mouse
Entrez Gene: 72161 Rat
IMAGE Id: 2958695 Human
Omim: 604884 Human
SwissProt: Q9HC98 Human
SwissProt: Q9ES70 Mouse
SwissProt: P59895 Rat
Unigene: 197071 Human
Unigene: 143818 Mouse
Unigene: 18744 Rat
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
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