Home
>
Product
>
Antibody
>
Rabbit Anti-phospho-Cdc25B (Ser323)/FITC Conjugated antibody
background:
CDC25B is a member of the CDC25 family of phosphatases. CDC25B activates the cyclin dependent kinase CDC2 by removing two phosphate groups and it is required for entry into mitosis. CDC25B shuttles between the nucleus and the cytoplasm due to nuclear localization and nuclear export signals. The protein is nuclear in the M and G1 phases of the cell cycle and moves to the cytoplasm during S and G2. CDC25B has oncogenic properties, although its role in tumor formation has not been determined. Multiple transcript variants for this gene exist. [provided by RefSeq, Jul 2008].
Function:
Tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. Required for G2/M phases of the cell cycle progression and abscission during cytokinesis in a ECT2-dependent manner. Directly dephosphorylates CDK1 and stimulates its kinase activity. The three isoforms seem to have a different level of activity.
Subunit:
Interacts with MAPK14 and 14-3-3 proteins.
Subcellular Location:
Cytoplasm, cytoskeleton, centrosome. Cytoplasm, cytoskeleton, spindle pole.
Post-translational modifications:
Phosphorylated by BRSK1 in vitro. Phosphorylated by CHEK1, which inhibits the activity of this protein. Phosphorylation at Ser-353 by AURKA might locally participate in the control of the onset of mitosis. Phosphorylation by MELK at Ser-169 promotes localization to the centrosome and the spindle poles during mitosis. Phosphorylation at Ser-323 and Ser-375 by MAPK14 is required for binding to 14-3-3 proteins.
Similarity:
Belongs to the MPI phosphatase family.
Contains 1 rhodanese domain.
Database links:
Entrez Gene: 994 Human
Entrez Gene: 12531 Mouse
Entrez Gene: 171103 Rat
Omim: 116949 Human
SwissProt: P30305 Human
SwissProt: P30306 Mouse
SwissProt: P48966 Rat
Unigene: 153752 Human
Unigene: 38444 Mouse
Unigene: 11312 Rat
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
|
|