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Rabbit Anti-CTCF/FITC Conjugated antibody
background:
CTCF (CCCTSLCbinding factor) is a highly conserved zinc finger protein that has been implicated in playing a role in a wide range of regulatory functions, including transcriptional activation/repression, insulation, imprinting, and X chromosome inactivation. A general role for CTCF in the global organisation of chromatin architecture has been proposed. It has been suggested that CTCF is involved, in a heritable manner, in the interplay between DNA methylation, higher-order chromatin structure, and lineage-specific gene expression.
Function:
Chromatin binding factor that binds to DNA sequence specific sites. Involved in transcriptional regulation by binding to chromatin insulators and preventing interaction between promoter and nearby enhancers and silencers. Acts as transcriptional repressor binding to promoters of vertebrate MYC gene and BAG1 gene. Also binds to the PLK and PIM1 promoters. Acts as a transcriptional activator of APP. Regulates APOA1/C3/A4/A5 gene cluster and controls MHC class II gene expression. Plays an essential role in oocyte and preimplantation embryo development by activating or repressing transcription. Seems to act as tumor suppressor. Plays a critical role in the epigenetic regulation. Participates in the allele-specific gene expression at the imprinted IGF2/H19 gene locus. On the maternal allele, binding within the H19 imprinting control region (ICR) mediates maternally inherited higher-order chromatin conformation to restrict enhancer access to IGF2. Plays a critical role in gene silencing over considerable distances in the genome. Preferentially interacts with unmethylated DNA, preventing spreading of CpG methylation and maintaining methylation-free zones. Inversely, binding to target sites is prevented by CpG methylation. Plays a important role in chromatin remodeling. Can dimerize when it is bound to different DNA sequences, mediating long-range chromatin looping. Mediates interchromosomal association between IGF2/H19 and WSB1/NF1 and may direct distant DNA segments to a common transcription factory. Causes local loss of histone acetylation and gain of histone methylation in the beta-globin locus, without affecting transcription. When bound to chromatin, it provides an anchor point for nucleosomes positioning. Seems to be essential for homologous X-chromosome pairing. May participate with Tsix in establishing a regulatable epigenetic switch for X chromosome inactivation. May play a role in preventing the propagation of stable methylation at the escape genes from X- inactivation. Involved in sister chromatid cohesion. Associates with both centromeres and chromosomal arms during metaphase and required for cohesin localization to CTCF sites. Regulates asynchronous replication of IGF2/H19.
Subunit:
Interacts with CHD8.
Subcellular Location:
Nucleus, nucleoplasm. Chromosome. Chromosome, centromere. Note=May translocate to the nucleolus upon cell differentiation. Associates with both centromeres and chromosomal arms during metaphase. Associates with the H19 ICR in mitotic chromosomes. May be preferentially excluded from heterochromatin during interphase.
Tissue Specificity:
Ubiquitous. Absent in primary spermatocytes.
Post-translational modifications:
Sumoylated on Lys-74 and Lys-689; sumoylation of CTCF contributes to the repressive function of CTCF on the MYC P2 promoter (By similarity).
Similarity:
Belongs to the CTCF zinc-finger protein family. Contains 11 C2H2-type zinc fingers.
Database links:
Entrez Gene: 10664 Human
Entrez Gene: 13018 Mouse
Entrez Gene: 83726 Rat
Omim: 604167 Human
SwissProt: P49711 Human
SwissProt: Q61164 Mouse
SwissProt: Q9R1D1 Rat
Unigene: 368367 Human
Unigene: 269474 Mouse
Unigene: 18612 Rat
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
CTCF是一种结构保守、功能多样的锌指蛋白。通过不同锌指的组合，识别不同的DNA序列，并且通过自身形成多聚体或与其它蛋白相互作用、与亚细胞核结构共定位等多种方式，在基因活化与沉默、基因印记、X-染色体失活以及基因组核内空间组织等不同生理过程中发挥关键作用。CTCF在细胞生长与增殖、系统发生以及肿瘤发生等方面都有着重要的作用。
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