Rabbit Anti-NIPP1/FITC Conjugated antibody

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Activator of RNA decay; ARD 1; ARD1; Homolog of E.coli RNase E; NIPP 1; NIPP1; Nuclear inhibitor of protein phosphatase 1; Nuclear subunit of PP1; PPP1R8; Protein phosphatase 1 regulatory inhibitor subunit 8; Protein phosphatase 1 regulatory subunit 8; RN

Cat:

SL3658R-FITC

Species Reactivity：

(predicted: Human,Mouse,Rat,Chicken,Pig,Cow,Horse,Rabbit,Guinea Pig,Daniorerio)

Immunogen：

KLH conjugated synthetic peptide derived from human NIPP1(25-75aa)

Format：

Lyophilized or Liquid

Storage instructions：

Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of ant

Buffer：

0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

IF=1:50-200not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Calculated MW：

38kDa

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Unit:

Price: $596.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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background:
Inhibitor subunit of the major nuclear protein phosphatase 1 (PP1). It has RNA binding activity but does not cleave RNA and may target PP1 to RNA associated substrates. May also be involved in pre mRNA splicing. Binds DNA and might act as a transcriptional repressor. Seems to be required for cell proliferation.

Function:
Inhibitor subunit of the major nuclear protein phosphatase-1 (PP-1). It has RNA-binding activity but does not cleave RNA and may target PP-1 to RNA-associated substrates. May also be involved in pre-mRNA splicing. Binds DNA and might act as a transcriptional repressor. Seems to be required for cell proliferation. Isoform Gamma is a site-specific single-strand endoribonuclease that cleaves single strand RNA 3' to purines and pyrimidines in A+U-rich regions. It generates 5'-phosphate termini at the site of cleavage. This isoform does not inhibit PP-1. May be implicated in mRNA splicing.

Subunit:
Interacts with phosphorylated CDC5L, SF3B1 and MELK. Interacts with EED, in a nucleic acid-stimulated manner. Part of a complex consisting of PPP1R8, EED, HDAC2 and PP-1. Part of the spliceosome. Interacts with PPP1CA, PPP1CB and PPP1CC.

Subcellular Location:
Nucleus. Nucleus speckle. Note=Primarily, but not exclusively, nuclear. Isoform Gamma: Cytoplasm. Note=Found mainly in the cytoplasm.

Tissue Specificity:
Ubiquitously expressed, with highest levels in heart and skeletal muscle, followed by brain, placenta, lung, liver and pancreas. Less abundant in kidney. The concentration and ratio between isoforms is cell-type dependent. Isoform Alpha (>90%) and isoform Beta were found in brain, heart and kidney. Isoform Gamma is mainly found in B-cells and T-lymphocytes, and has been found in 293 embryonic kidney cells.

Post-translational modifications:
May be inactivated by phosphorylation on Ser-199 or Ser-204. Phosphorylated by Lyn in vitro on Tyr-264, and also on Tyr-335 in the presence of RNA.

Similarity:
Contains 1 FHA domain.

Database links:

Entrez Gene: 5511 Human

Entrez Gene: 100336 Mouse

Entrez Gene: 313030 Rat

Omim: 602636 Human

SwissProt: Q12972 Human

SwissProt: Q8R3G1 Mouse

Unigene: 533474 Human

Unigene: 105230 Mouse

Unigene: 16580 Rat

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

NIPP1是一种普遍存在于细胞核中的蛋白，它在剪接体的装配中发挥着重要作用，主要体现在细胞的分化，增值及迁移中。经研究，目前NIPP1在乳腺肿瘤、前列腺癌,以及肺癌中有较高频率的表达,其中乳腺肿瘤中的表达频率最高,达到70%,远高于其他肿瘤组织。表明ARD1蛋白的高表达可能是乳腺肿瘤组织的一个标志。

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