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Rabbit Anti-Phospho-Vimentin (Ser56)/FITC Conjugated antibody
background:
Vimentin is the major subunit protein of the intermediate filaments of mesenchymal cells. It is believed to be involved with the intracellular transport of proteins between the nucleus and plasma membrane. Vimentin has been implicated to be involved in the rate of steroid synthesis via its role as a storage network for steroidogenic cholesterol containing lipid droplets. Vimentin phosphorylation by a protein kinase causes the breakdown of intermediate filaments and activation of an ATP and myosin light chain-dependent contractile event. This results in cytoskeletal changes that facilitate the interaction of the lipid droplets within mitochondria, and subsequent transport of cholesterol to the organelles leading to an increase in steroid synthesis. Immunohistochemical staining for Vimentin is characteristic of sarcomas (of neural, muscle and fibroblast origin) compared with carcinomas which are generally negative. Melanomas, lymphomas and vascular tumors may all stain for Vimentin. Vimentin antibodies are thus of value in the differential diagnosis of undifferentiated neoplasms and malignant tumors. They are generally used with a panel of other antibodies including those recognizing cytokeratins, lymphoid markers, S100, desmin and neurofilaments.
Function:
Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally.
Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2.
Subunit:
Homopolymer. Interacts with HCV core protein. Interacts with LGSN and SYNM. Interacts (via rod region) with PLEC (via CH 1 domain).Interacts with SLC6A4. Interacts with STK33. Interacts with LARP6. Interacts with RAB8B.
Subcellular Location:
Cytoplasm.
Tissue Specificity:
Highly expressed in fibroblasts, somev expression in T- and B-lymphocytes, and little or no expression in Burkitt's lymphoma cell lines. Expressed in many hormone-independent mammary carcinoma cell lines.
Post-translational modifications:
Filament disassembly during mitosis is promoted by phosphorylation at Ser-55 as well as by nestin (By similarity). One of the most prominent phosphoproteins in various cells of mesenchymal origin. Phosphorylation is enhanced during cell division, at which time vimentin filaments are significantly reorganized. Phosphorylation by PKN1 inhibits the formation of filaments. Phosphorylated at Ser-56 by CDK5 during neutrophil secretion in the cytoplasm. Phosphorylated by STK33.
Similarity:
Belongs to the intermediate filament family.
Database links:
Entrez Gene: 7431 Human
Entrez Gene: 22352 Mouse
Entrez Gene: 81818 Rat
Omim: 193060 Human
SwissProt: P08670 Human
SwissProt: P20152 Mouse
SwissProt: P6200 Rat
Unigene: 455493 Human
Unigene: 691131 Human
Unigene: 53600 Mouse
Unigene: 2710 Rat
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
Vimentin—波形蛋白。是五种主要的中间丝之一,存在于各种正常和病理性间质来源的组织,如纤维母细胞、内皮细胞、淋巴细胞等正常细胞和肉瘤、淋巴瘤、黑色素瘤等肿瘤。波形蛋白是负责维持细胞骨架完整性的蛋白之一。
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