background:
NuMA (Nuclear Mitotic Apparatus Protein) is an intranuclear protein and present in nucleus during interphase. At the onset of mitosis, it redistributes from the nucleus to two centrosomal structures that later will become part of the mitotic spindle pole. After anaphase, the protein redistributes from the spindle polar region into reforming nucleus. NuMA is an essential protein during mitosis for the terminal phases of chromosome separation and/or nuclear reassembly. Recently a study shows that NuMA is cleaved to a 36 to 200kDa during apoptosis.
Function:
Highly abundant component of the nuclear matrix where it may serve a non-mitotic structural role, occupies the majority if the nuclear volume. Required for maintenance and establishment of the mitotic spindle poles, functionning as a tether linking bulk microtubules of the spindle to centrosomes. May be involved in coordination of the alignment of the mitotic spindle to the cellular polarity axis, which is a prerequisite for asymmetric cell divisions.
Subunit:
Homodimer. Also forms multiarm oligomers by association of SLCterminal tail domains, oligomers may further assemble to form a hexagonal nuclear lattice-like network. Interacts with TNKS.
Subcellular Location:
Nucleus matrix. Chromosome. Cytoplasm, cytoskeleton, spindle pole. Note=Resides in the nuclear matrix during interphases. Dissociates from condensing chromosomes during early prophase, and relocates to the spindle poles via dynein/dynamin association, it remain there until the anaphase onset. Before the complete disintegration of the nuclear lamina. As mitosis progresses it reassociates with telophase chromosomes very early during nuclear reformation, before substantial accumulation of lamins on chromosomal surfaces is evident.
Isoform Numa-m: Cytoplasm. Note=Mainly clustered at the centrosomal region.
Isoform Numa-s: Cytoplasm. Note=Mainly clustered at the centrosomal region.
Post-translational modifications:
ADP-ribosylated by TNKS during mitosis.
Phosphorylated in the SLCterminal tail during mitosis, probably by CDK1. Phosphorylation increases solubility and promotes association with dynein and subsequent translocation to the spindle poles.
Database links:
Entrez Gene: 4926 Human
Entrez Gene: 101706 Mouse
Entrez Gene: 308870 Rat
Omim: 112809 Human
SwissProt: Q14196 Human
SwissProt: Q3TH77 Mouse
SwissProt: Q4G051 Rat
Unigene: 325978 Human
Unigene: 66392 Rat
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
NuMA (Nuclear mitotic apparatus protein)是细胞核基质中的一种高分子量蛋白质，具有一个特殊的细胞周期依赖性.核有丝分裂器蛋白NuMA与人类肿瘤细胞的恶性增殖相关.
|
|
