Rabbit Anti-MLK3/FITC Conjugated antibody

Home > Product > Antibody > Rabbit Anti-MLK3/FITC Conjugated antibody

MEKK11; MGC17114; Mitogen-activated protein kinase kinase kinase 11; Mixed lineage kinase 3; Mixed lineage protein kinase 3; Mlk-3; Mlk3; Protein tyrosine kinase PTK1; PTK1; RHOE; SH3 domain containing proline rich kinase; SPRK; Src-homology 3 domain-co

Cat:

SL2992R-FITC

Species Reactivity：

Human,Mouse,Rat,(predicted: Cow,Horse,)

Immunogen：

KLH conjugated synthetic peptide derived from human MLK3

Format：

Lyophilized or Liquid

Storage instructions：

Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of ant

Buffer：

0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

IF=1:50-200not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Calculated MW：

93kDa

prev next

Unit:

Price: $596.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
Feedback Wall

background:
Members of the mixed-lineage kinase (MLK) family (including MLK1, MLK2, MLK3, and dual leucine zipper kinase [DLK]) are serine/threonine protein kinases that are expressed in multiple cell types. MLK3 is activated by phosphorylation in response to stress stimuli (e.g., inflammatory responses, UV, chemical stress) that are coupled to the small GTPase, Cdc42/rac. MLK3 is a multifunctional kinase that plays an essential role in several signaling pathways, including mitogen-activated protein kinase (i.e. activation of JNK and p38), IkappaB/NFkappaB, and p70 S6 kinase. Indeed MLK3 signaling occurs through multiple signaling domains in this protein kinase including (from N- to SLCterminal) a glycine-rich domain, Src homology 3 (SH3) domain, a kinase domain, a zipper domain, a Cdc42/rac interactive binding (CRIB) domain and a Pro/Ser/Thr-rich domain. Phosphorylation of MLK3 occurs on multiple residues including threonine 277 and serine 281 within the activation loop of the kinase domain.

Function:
Activates the JUN N-terminal pathway. Required for serum-stimulated cell proliferation and for mitogen and cytokine activation of MAPK14 (p38), MAPK3 (ERK) and MAPK8 (JNK1). Plays a role in mitogen-stimulated phosphorylation and activation of BRAF, but does not phosphorylate BRAF directly. Influences microtubule organization during the cell cycle.

Subunit:
Homodimer; undergoes dimerization during activation.

Subcellular Location:
Cytoplasm, cytoskeleton, centrosome. Note=Location is cell cycle dependent.

Tissue Specificity:
Expressed in a wide variety of normal and neoplastic tissues including fetal lung, liver, heart and kidney, and adult lung, liver, heart, kidney, placenta, skeletal muscle, pancreas and brain.

Post-translational modifications:
Autophosphorylation on serine and threonine residues within the activation loop plays a role in enzyme activation. Thr-277 is likely to be the main autophosphorylation site. Phosphorylation of Ser-555 and Ser-556 is induced by CDC42.

Similarity:
Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.
Contains 1 protein kinase domain.
Contains 1 SH3 domain.

Database links:

Entrez Gene: 4296 Human

Entrez Gene: 21283 Mouse

Omim: 600050 Human

SwissProt: Q16584 Human

SwissProt: Q6P2G4 Human

SwissProt: Q80XI6 Mouse

Unigene: 502872 Human

Unigene: 185026 Mouse

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

MLK3是JNK蛋白上游的重要活化因子，通过直接磷酸化并激活中游MKK4／7蛋白激酶，从而激活JNK应激信号通路。

Product Feedback Wall