Rabbit Anti-Phospho-KSR1 (Ser392)/FITC Conjugated antibody

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KSR1 (phospho S392); p-KSR1 (phospho S392); BKSR 1; BKSR1; dKsr; EK 31; EK31; hb; Kinase suppressor of Ras 1; Kinase suppressor of ras; KSR 1; KSR; KSR1; KSR1_HUMAN; Positive Ras signaling mediator family member (86.4 kD); Positive Ras signa

Cat:

SL348R-FITC

Species Reactivity：

(predicted: Human,Mouse,Rat,)

Immunogen：

KLH conjugated synthesised phosphopeptide derived from human KSR1 around the phosphorylation site of Ser392(390-394aa)

Format：

Lyophilized or Liquid

Storage instructions：

Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of ant

Buffer：

0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

IF=1:50-200not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Calculated MW：

102kDa

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Unit:

Price: $596.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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background:
KSR1 is a molecular scaffold and positive regulator of the Raf/MEK/ERK phosphorylation cascade. It was initially considered to be a protein kinase acting in the Ras pathway. KSR1 is required for maximal ERK activation induced by growth factors and by some cytotoxic agents. In resting cells, phosphorylated KSR1 is cytoplasmic and in stimulated cells, dephosphorylated KSR1 is membrane associated.

Function:
Location-regulated scaffolding protein connecting MEK to RAF. Promotes MEK and RAF phosphorylation and activity through assembly of an activated signaling complex. By itself, it has no demonstrated kinase activity.

Subunit:
Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. Contains 1 phorbol-ester/DAG-type zinc finger. Contains 1 protein kinase domain.

Subcellular Location:
Cytoplasm. Membrane. In unstimulated cells, where the phosphorylated form is bound to a 14-3-3 protein, sequestration in the cytoplasm occurs. Following growth factor treatment, the protein is free for membrane translocation, and it moves from the cytoplasm to the cell periphery.

Post-translational modifications:
Phosphorylated on Ser-309 and, to a higher extent, on Ser-404 by MARK3. Dephosphorylated on Ser-404 by PPP2CA. In resting cells, phosphorylated KSR1 is cytoplasmic and in stimulated cells, dephosphorylated KSR1 is membrane-associated (By similarity).

Similarity:
Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.
Contains 1 phorbol-ester/DAG-type zinc finger.
Contains 1 protein kinase domain.

Database links:

Entrez Gene: 8844 Human

Entrez Gene: 16706 Mouse

Entrez Gene: 72573 Rat

Omim: 601132 Human

SwissProt: Q8IVT5 Human

SwissProt: Q61097 Mouse

Unigene: 133534 Human

Unigene: 4745 Mouse

Unigene: 179021 Rat

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

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