Rabbit Anti-CD80/FITC Conjugated antibody

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Activation B7-1 antigen; B lymphocyte activation antigen B7; B7; B7-1; B7-1 antigen; BB1; CD28 antigen ligand 1; CD28LG; CD28LG1; CD80; CD80 antigen (CD28 antigen ligand 1, B7-1 antigen); CD80 antigen; CD80 molecule; CD80_HUMAN; Costimulator

Cat:

SL2211R-FITC

Species Reactivity：

Mouse,(predicted: Rat,)

Immunogen：

KLH conjugated synthetic peptide derived from rat CD80

Format：

Lyophilized or Liquid

Storage instructions：

Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of ant

Buffer：

0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

Flow-Cyt=0.2ug/TestICC=1:50-200IF=1:50-200not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Calculated MW：

30kDa

Product Overview：

Blank control: Mouse spleen. Primary Antibody (green line): Rabbit Anti-CD80 antibody (SL2211R-FITC) Dilution: 0.2μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . ProtocolThe cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20,000 events was performed.

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Unit:

Price: $596.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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background:
CD80 is a member of the Ig superfamily and serves as the ligand for two T cell molecules, CD28 and CTLA4. Interactions between CD28 and CD80 on activated B cells result in enhanced T cell activation. CD80 is rapidly induced on the surface of in vitro activated B cells, Epstein Barr Virus (EBV) transformed B cell lines, Burkitts lymphoma cell lines, freshly isolated follicular B lymphoma cells, T cells, and monocytes. It is also expressed at high levels in dendritic cells. It reacts weakly with a small proportion of non activated normal B cells and with HTLV1 infected T cells. CD80 does not react with peripheral monocytes, resting and activated normal T cells, T cell lines and T cell clones, nor with myelomonocytic cell lines.

Function:
Involved in the costimulatory signal essential for T lymphocytes activation. T-cell proliferation and cytokine production is induced by the binding of CD28 or CTLA-4 to this receptor.

Subcellular Location:
Membrane; Single-pass type I membrane protein.

Tissue Specificity:
Expressed on activated B-cells, macrophages and dendritic cells.

Similarity:
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 1 Ig-like SLVtype (immunoglobulin-like) domain.

Database links:

Entrez Gene: 941 Human

Entrez Gene: 12519 Mouse

Omim: 112203 Human

SwissProt: P33681 Human

SwissProt: Q00609 Mouse

Unigene: 838 Human

Unigene: 89474 Mouse

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. Picture

Blank control: Mouse spleen.
Primary Antibody (green line): Rabbit Anti-CD80 antibody (SL2211R-FITC)
Dilution: 0.2μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Protocol
The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20,000 events was performed.

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