Rabbit Anti-CD30/FITC Conjugated antibody

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CD 30; CD30; CD30 antigen; CD30L receptor; Cytokine receptor CD30; D1S166E; KI 1; Ki 1 antigen; Ki-1 antigen; KI1; Lymphocyte activation antigen CD30; TNFRSF 8; TNFRSF8; TNFRSF8; TNR8_HUMAN; Tumor Necrosis Factor Receptor Superfamily Member 8; Tumor Necro

Cat:

SL2495R-FITC

Species Reactivity：

Human,Pig,(predicted: Rabbit,)

Immunogen：

KLH conjugated synthetic peptide derived from human CD30

Format：

Lyophilized or Liquid

Storage instructions：

Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of ant

Buffer：

0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

Flow-Cyt=2ug/TestIF=1:50-200not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Calculated MW：

62kDa

Product Overview：

Blank control: K562. Primary Antibody (green line): Rabbit Anti-TNFRSF8 antibody (SL2495R-FITC) Dilution: 2μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . ProtocolThe cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with PBST for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature.Acquisition of 20,000 events was performed.

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Unit:

Price: $596.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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background:
The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor is expressed by activated, but not by resting, T and B cells. TRAF2 and TRAF5 can interact with this receptor, and mediate the signal transduction that leads to the activation of NF-kappaB. This receptor is a positive regulator of apoptosis, and also has been shown to limit the proliferative potential of autoreactive CD8 effector T cells and protect the body against autoimmunity. Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008]

Function:
Receptor for TNFSF8/CD30L. May play a role in the regulation of cellular growth and transformation of activated lymphoblasts. Regulates gene expression through activation of NF-kappa-B.

Subunit:
Interacts with TRAF1, TRAF2, TRAF3 and TRAF5.

Subcellular Location:
Isoform Long: Cell membrane; Single-pass type I membrane protein.
Isoform Short: Cytoplasm.

Post-translational modifications:
Phosphorylated on serine and tyrosine residues.

Similarity:
Contains 6 TNFR-Cys repeats.

Database links:

Entrez Gene: 943 Human

Omim: 153243 Human

SwissProt: P28908 Human

Unigene: 1314 Human

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

CD30是一种单链糖蛋白，标记活化T和B淋巴细胞，R-S细胞和部分淋巴滤泡间区免疫母细胞。CD30是霍奇金淋巴瘤的一个特异性标记，也是大细胞间变型淋巴瘤的诊断型标志。 Picture

Blank control: K562.
Primary Antibody (green line): Rabbit Anti-TNFRSF8 antibody (SL2495R-FITC)
Dilution: 2μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with PBST for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature.Acquisition of 20,000 events was performed.

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